Cucumber seedling substrate and preparing method thereof
A technology for raising seedlings and cucumbers, which is applied in the preparation, application, organic fertilizers and other directions of organic fertilizers, can solve the problems of non-uniformity and non-renewability of peat resource distribution, damage to the balance of wetland ecosystems, and increase of seedling raising costs. The effect of non-renewable problems, promoting growth and reducing the occurrence of soil-borne diseases
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Embodiment 1
[0022] Example 1 Growth promotion effect of Bacillus amyloliquefaciens
[0023] 1. Activation and fermentation culture of Bacillus amyloliquefaciens
[0024] Transfer the Bacillus amyloliquefaciens WS-8 stored on the slope to a 50mL Erlenmeyer flask for seed culture
[0025] After culturing overnight, it is transferred to the fermentation medium for culture after microscopic examination of no bacteria.
[0026] The liquid fermentation medium formula of Bacillus amyloliquefaciens is: starch 0.7%, soybean meal powder 1.4%, sucrose 0.2%, yeast extract 0.06%, peptone 0.05%, ammonium sulfate 0.13%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.03% , Manganese sulfate 0.01%, sodium chloride 0.02%, calcium carbonate 0.1%, pH 7.3-7.5, the number of spores can reach 12 billion / ml after 48 hours of cultivation.
[0027] 2. Growth promotion effect of Bacillus amyloliquefaciens
[0028] The experiment set up three treatments: blank control (water irrigation), fermentation broth irrigati...
Embodiment 2
[0032] Example 2 Preparation of biochar
[0033] The fermented Bacillus amyloliquefaciens fermentation broth was adsorbed with straw-derived biochar at 15%, 20% and 25% mass addition ratios, and the number of Bacillus amyloliquefaciens in the adsorbed biochar was checked regularly. Table 2 shows that the adsorption capacity of 20% is the best, and the number of live spores is the most retained. The number of live spores stored for 6 months is relatively stable, and the adsorption capacity is not easy to be infected with bacteria.
[0034] Table 2 The shelf life of different addition ratios
[0035] .
Embodiment 3
[0036] Example 3 Determination of the volume ratio of organic fertilizer, biomass charcoal inoculum, and straw powder
[0037] Mix organic fertilizer (biogas residue + chicken manure, where the mass ratio of biogas residue: chicken manure = 1:2.5), biochar agent (absorption capacity of Bacillus amyloliquefaciens is 20%) and straw powder in different mass ratios It can be seen from Table 3 that the mass ratio of treatment 3, treatment 4, and treatment 5 can effectively increase the germination index and strong seedling index of cucumber, and the difference between treatments is not significant. Based on the consideration of input cost, organic fertilizer, The best quality ratio of biochar and straw powder is 7:40:2, which can effectively increase cucumber germination index and strong seedling index.
[0038] Table 3 Effects of different components on germination index and strong seedling index
[0039]
[0040] Note: The lowercase letters in the table represent significant difference...
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