A kind of Escherichia coli engineering bacteria producing 5-aminolevulinic acid

A technology of aminolevulinic acid and Escherichia coli, applied in the fields of metabolic engineering and microbial fermentation, can solve the problems of high cost and unsuitable for large-scale industrial production, and achieve the effects of reducing production cost and shortening fermentation cycle.
CN106497858BActive Publication Date: 2019-06-21JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2019-06-21

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Abstract

The invention discloses engineered escherichia coli for producing 5-aminolevulinic acid and belongs to the fields of metabolic engineering and microbial fermentation. The engineered escherichia coli is characterized by carrying out co-expression of a gene coaA for coding pantothenate kinase, a gene dfp for coding phosphoric acid pantothenylcysteine synthetase and decarboxylase and a gene coaD for coding phosphoric acid CoA pyrophosphorylase from an escherichia coli CoA synthetic pathway by using pUC19 and pETDuet-1 vectors on the basis of excess expression of 5-aminolevulinate synthase hemA which is used as a key enzyme in a synthetic pathway of 5-aminolevulinic acid C4 by using a vector pRSFDuet-1-hemA to build recombination strains. Shake-flask culture proves that the yield of recombinant bacteria ALA for co-expression of CoA pathway genes is obviously improved; the maximum yield reaches 700mg / L and is increased by about 46% in comparison with contrast.
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Description

technical field

[0001] The invention relates to an Escherichia coli engineering bacterium for producing 5-aminolevulinic acid, belonging to the fields of metabolic engineering and microbial fermentation. Background technique

[0002] 5-aminolevulinic acid (5-aminolevulinic acid, ALA), the molecular formula is C 5 o 3 NH 9 , with a molecular weight of 131.13 and a melting point of 149-151°C, it is the biosynthesis of chlorophyll, heme, and vitamin B 12 and other key precursor substances. As a safe, selective, and permeable photodynamic drug, ALA has gradually attracted attention in the medical field, and has been successfully used in the diagnosis and photodynamic therapy of skin cancer, bladder cancer, digestive tract cancer, and lung cancer. In addition, as a new photoactivated pesticide with high environmental compatibility and high selectivity, ALA is widely used in the field of pesticides, such as a pollution-free green pesticide, herbicide and plant growth regulator...

Claims

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