Method for producing chitooligo saccharide via acidolysis and enzymolysis by taking shrimp and crab shells as raw material

Abstract

The invention provides a method for producing chitooligo saccharide via acidolysis and enzymolysis by taking shrimp and crab shells as a raw material. The method comprises the following steps: immersing the shrimp and crab shells in water for 1-2 days, drying and smashing, screening the shrimp and crab shells with a sieve of 20 meshes, adding the sieved shrimp and crab shells into a hydrochloric acid aqueous solution according to the standard that every 100 g of the shrimp and crab shells correspond to 1,000-1,100 ml of the hydrochloric acid aqueous solution, rising the temperature to 75-85 DEG C, adding mixed bacteria, and carrying out reaction for 6-7 hours; then adding potassium hydroxide till the pH value is greater than 8, freezing for 2-3 hours, then melting the product, adding a sodium chloride aqueous solution for mixing, and carrying out filtering and vacuum drying to obtain the chitooligo saccharide. By the adoption of enzymatic degradation, high-concentration conversion of a high-viscosity substrate is realized. The problem of conversion of the high-concentration substrate is effectively solved, and the production cost is substantially reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing chitosan oligosaccharide by acidolysis and enzymolysis using shrimp and crab shells as raw materials. Background technique [0002] Chitosan is the deacetylation product of chitin. Chitin widely exists in crustaceans such as shrimps and crabs, as well as insects, algae and bacteria. It is the second largest natural polymer compound after cellulose in the world, and its annual biosynthesis amount reaches billions of tons. Due to its inactive chemical properties and poor solubility, chitin has limited direct application; however, the chitosan obtained after deacetylation of chitin has poor solubility, The chemical properties have been greatly changed, and it has a wide range of application values ​​in food, medicine, daily chemicals, environmental protection, agriculture, etc. The molecular weight of chitosan macromolecules is usually around hundred...

AI Technical Summary

Problems solved by technology

Specific chitosanase can specifically degrade chitosan, but current research shows that its degradation efficiency is not very high, resulting in higher production costs

Non-specific enzymatic hydrolysis has been studied by many people. The literature "Journal of Wuxi University of Light Industry, 1996 No. 15" reported that Professor Xia Wenshui of Wuxi University of Light Industry used wheat germ lipase to degrade chitosan to obtain low chitosan with an average molecular weight of tens of thousands. The literature "Carbohydr.Res.1992,237:325" reported that Pantaleone et al. studied the degradation of chitosan by cellulase, papain, and dextranase under certain conditions, but the result only obtained an average molecular weight of Tens of thousands of low chitosan, but cannot degrade chitosan into lower molecular weight chitooligosaccharides