Pseudomonas aeruginosa and application thereof
A technology of Pseudomonas aeruginosa and milk powder, applied in Pseudomonas aeruginosa and its application field, can solve problems such as protein allergy
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Embodiment 1
[0013] The screening method of embodiment 1 Pseudomonas aeruginosa (CCTCC NO:M 2016533)
[0014] Weigh 1.0g mixed soil sample and 5mL sterile water and mix it in a 10mL centrifuge tube. After shaking and mixing, use sterile water to dilute 10, 100, and 1000 times respectively, then apply skimmed milk powder plate medium, and then place in a constant temperature incubator Incubate at 30°C for 24 hours.
[0015] Select the colony produced by the hydrolysis circle and transfer it to a new skimmed milk powder plate medium, and then culture it statically at 30°C for 24 hours in a constant temperature incubator. Then observe the size of the hydrolysis circle and the shape of the colony, and transfer the single colony S8 with the largest hydrolysis circle to LB medium for activation for 8 hours, then transfer it to LB medium containing 20% glycerol, and store it at -80 ℃.
[0016] The 16S rRNA sequence of the strain S8 is shown as SEQ ID NO.1, and the strain identification result...
Embodiment 2
[0017] α of embodiment 2 Pseudomonas aeruginosa S8 (CCTCC NO: M 2016533) fermented liquid S1 - Casein removal efficiency
[0018] The starting strain Pseudomonas aeruginosa (CCTCC NO:M 2016533) stored in a glycerol tube at -80°C was inserted into LB medium at an inoculum size of 2%, and cultured at 30°C and 220rpm for 12h.
[0019] The activated Pseudomonas aeruginosa was transferred to fresh LB medium with 2% inoculation amount, and the fermentation broth was collected after culturing at 30° C. and 220 rpm for 12 hours, and the supernatant of the fermentation broth was collected by centrifugation at 8000 rpm.
[0020] The fermentation supernatant was mixed with α S1 - After the casein standard was mixed at 1:1, react at 30°C for 1 hour. with alpha S1 -The casein standard was used as a control, and the CSN1S8 enzyme-linked reaction kit was used to detect the α S1 - Casein content.
[0021] Add 50 μl of samples to the bottom of the wells of the microplate, try not to touch...
Embodiment 3
[0024] Example 3 Pseudomonas aeruginosa S8 (CCTCC NO: M 2016533) fermentation broth β-lactoglobulin removal efficiency
[0025] The starting strain Pseudomonas aeruginosa (CCTCC NO:M 2016533) stored in a glycerol tube at -80°C was inserted into LB medium at an inoculum size of 2%, and cultured at 30°C and 220rpm for 12h.
[0026] The activated Pseudomonas aeruginosa was transferred to fresh LB medium with 2% inoculation amount, and the fermentation broth was collected after culturing at 30° C. and 220 rpm for 12 hours, and the supernatant of the fermentation broth was collected by centrifugation at 8000 rpm.
[0027] The supernatant of the fermentation broth was mixed with the β-lactoglobulin standard at a ratio of 1:1, and reacted at 30°C for 1 hour. Using the β-lactoglobulin standard as a control, use the CSN1S8 enzyme-linked reaction kit to detect the α of the samples respectively. S1 - Casein content.
[0028] Add 50 μl of samples to the bottom of the wells of the microp...
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