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Inducing method of staphylococcus aureus in viable but nonculturable state

A Staphylococcus aureus technology, applied in the biological field, can solve the problems of high process energy consumption, environmental burden, long time Staphylococcus aureus bacteria, etc., to achieve the effect of promoting energy saving, reducing consumption and shortening induction time

Inactive Publication Date: 2017-03-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing research results show that it takes a relatively long time to obtain a large proportion of live Staphylococcus aureus bacteria in a non-culturable state, and the process consumes high energy, which is likely to cause a burden on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The rapid induction of the non-culturable state Staphylococcus aureus of embodiment 1 live

[0025] - Bacterial Suspension Preparation

[0026] The Staphylococcus aureus standard strain ATCC 25923 was inoculated in ordinary broth medium, and cultured on a shaker at 37°C until the logarithmic growth phase. Adopt 0.85% (mass percentage composition) aseptic normal saline to wash the thalli of logarithmic growth phase twice, resuspend, make thalline concentration be about 10 8 CFU / mL, as the bacterial liquid (bacterial suspension) to be induced;

[0027] Two low temperature plasma treatment

[0028] Experimental group: The Staphylococcus aureus suspension was treated with a dielectric barrier discharge low-temperature plasma sterilization device (Nanjing Suman Plasma Technology Co., Ltd.), and the specific steps were: put 3mL of the bacterial suspension into a quartz petri dish (50mm in diameter) , put the petri dish between the two electrodes, and carry out low-temperat...

Embodiment 2

[0038] Example 2 Rapidly induces a large proportion of Staphylococcus aureus to form a live non-culturable state

[0039] - Bacterial Suspension Preparation

[0040] It is the same as Step 1 in Example 1.

[0041] Two low temperature plasma induction

[0042] The method of step 2 in Example 1 is basically the same, only the treatment power is changed to 50W to obtain the induced bacterial liquid.

[0043] Three detection

[0044] Same as the third method in Example 1.

[0045] The results are as follows: the ratio of viable bacteria after dielectric barrier discharge low-temperature plasma treatment was 95.72%; the ratio of cultivable bacteria was 23.17%; In the control group, the ratio of viable bacteria to cultivable bacteria was still about 100%, and it was basically considered that there were no viable bacteria in a non-culturable state. It can be seen that the method of the present invention obtains a large proportion of live Staphylococcus aureus in a non-culturable...

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PUM

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Abstract

The invention discloses an inducing method of staphylococcus aureus in the viable but nonculturable state. The inducing method comprises the following steps: preparing staphylococcus aureus into a cell suspension, and carrying out dielectric barrier discharge low-temperature plasma treatment, thus forming the viable but nonculturable state, wherein the conditions of the dielectric barrier discharge low-temperature plasma treatment are as follows: the treatment frequency is 8-15 kHz, the treatment air gap is 1-3 mm, the treatment power is 25-50 W, the treatment time is 3-5 s, and the treatment temperature is room temperature. According to the method provided by the invention, staphylococcus aureus is subjected to dielectric barrier discharge low-temperature plasma treatment, so that the staphylococcus aureus can form the viable but nonculturable state within a short time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-temperature plasma induction method for live non-culturable Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is one of the four major food-borne pathogens that threaten human health. It can produce enterotoxins that cannot be destroyed by high temperature, thus causing human health hazards. In food, food that is easily contaminated by Staphylococcus aureus mainly includes fish, meat, eggs, milk and their products, especially the safety of dairy products is often threatened by Staphylococcus aureus, food poisoning incidents caused by Staphylococcus aureus every year It happens, it's a worldwide health problem. And the study found that in the actual production and distribution process of dairy products, due to technical defects, improper human operation, and external adverse environmental stress and other factors, sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N1/20C12R1/445
CPCC12N13/00C12N1/20
Inventor 刘东红丁甜李娇廖新浴索原杰叶兴乾陈健初胡亚芹陈士国
Owner ZHEJIANG UNIV
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