Method for using ultra-high performance liquid chromatography-quadrupole electrostatic field ion trap mass spectrum to screen vitamin and natural antioxidant in health care product

Abstract

The invention discloses a method for using an ultra-high performance liquid chromatography-quadrupole electrostatic field ion trap mass spectrum to screen vitamin and natural antioxidant in a health care product, and belongs to the technical field of food safety inspection. The method comprises the steps of firstly conducting extraction and purification on a sample using an optimized QuEChERS method; secondly conducting screening through a Q-Orbitrap, adopting three scan patterns of electrospray ion source and full scan / data dependence acquisition, full scan / variable data independence acquisition, full scan / full ion fragmentation acquisition, and obtaining integrated primary mass spectrum and secondary mass spectrum ; then adopting Exact Finder 2.5 software to conduct treatment on the obtained primary mass spectrum and secondary mass spectrum to extract information of a compound. By comparing information in an established spectrum library and information of screening results of the detected, constitution of vitamin and natural antioxidant in a green tea health care product is confirmed. The method for using the ultra-high performance liquid chromatography-quadrupole electrostatic field ion trap mass spectrum to screen vitamin and natural antioxidant in the health care product has the advantages that resolution is high, qualitative and quantitative results are accurate, sensitivity is high, quality accuracy is high and the like, and can be applicable to analysis of a complex substrate. The screening method has a very good application prospect in food industry.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of food safety detection, and in particular relates to an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for vitamins and natural antioxidants in health care products. [0003] 【Background technique】 [0004] In the United States, green tea is used as a dietary supplement, due to its rich bioactive substances, including caffeine, amino acids, vitamins, etc., so as to achieve the effect of promoting human health. Green tea extract, like vitamins and natural antioxidants, is often used to prevent and treat malnutrition, weight loss, and lower cholesterol levels, as well as protect the skin from sun damage. Green tea extract is often sold as a nutraceutical in tablet or capsule form. At present, nutraceuticals have become a part of the daily diet to make up for malnutrition caused by lifestyle, reduce the incidence of dise...

AI Technical Summary

Problems solved by technology

[0005] At present, there are few high-throughput and rapid detection methods for exogenous risk substances in health products, the pretreatment operation is complicated, the cost of materials and equipment is high, and it is limited to the rapid detection of several or one type of compounds

Vitamins and natural antioxidants have different structures and chemical properties, and it is difficult to detect and analyze both substances at the same time

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