Quantum dot-antibody fluorescent probe, preparation method, probe and test paper strip

A fluorescent probe and quantum dot technology, applied in the detection field, can solve the problems of high toxicity, low luminous efficiency, poor uniformity and dispersion, etc., and achieve the effects of high detection sensitivity, high fluorescence recovery rate, and high detection accuracy
CN106526192AInactive Publication Date: 2017-03-22JIANGSU LIANGDIAN TECH CO LTD
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
JIANGSU LIANGDIAN TECH CO LTD
Publication Date
2017-03-22
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention provides a quantum dot-antibody fluorescent probe preparation method, a quantum dot-antibody fluorescent probe, and an immunochromatography test paper strip and a preparation method thereof. The quantum dot-antibody fluorescent probe preparation method comprises: (1) adding quantum dots to a borate buffer solution to prepare a quantum dot solution; (2) adding an activator to the quantum dot solution, and activating, wherein the activator is 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or a hydrochloride thereof and N-hydroxysuccinimide or a sulfide thereof, a molar ratio of the activator to the quantum dots is 5-9:1, and a molar ratio of the 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or the hydrochloride thereof to the N-hydroxysuccinimide or the sulfide thereof is 2-3:4-5; and 3) adding antibody to the activated quantum dot solution, and coupling to obtain the quantum dot-antibody fluorescent probe, wherein a molar ratio of the antibody to the quantum dots is 4-2:1.
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Description

Technical field

[0001] The invention relates to the field of detection, in particular to a method for preparing a quantum dot-antibody fluorescent probe, a quantum dot-antibody fluorescent probe, and an immunochromatographic test strip containing the probe. Background technique

[0002] Quantum dots (QDs), also known as semiconductor nanocrystals, are usually composed of II-VI and III-V elements, with adjustable size, wide excitation spectrum, narrow emission spectrum, large Stoke shift, etc. Features. The molar absorption coefficient and fluorescence quantum yield of quantum dots are relatively high, which can generate strong fluorescence signals and improve detection sensitivity. At the same time, quantum dots have good photochemical stability and good photobleaching resistance, which improves the stability of detection results. In summary, quantum dots are an ideal fluorescent probe material, and have unique advantages and broad application prospects in the detection field b...

Claims

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