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Feeding method for improving fermentation growth efficiency of butyric acid bacteria

A technology of butyric acid bacteria and growth rate, applied in the field of agricultural biology, to achieve the effects of low cost, high controllability of operation and short fermentation time

Active Publication Date: 2017-04-26
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art does not apply the exponential feeding mode to the report of the anaerobic microorganism-butyric acid bacteria fermentation feeding process

Method used

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  • Feeding method for improving fermentation growth efficiency of butyric acid bacteria
  • Feeding method for improving fermentation growth efficiency of butyric acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of culture medium

[0023] Glucose 20g / L, peptone 30g / L, yeast powder 5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, light calcium carbonate 1g / L, manganese sulfate monohydrate 0.02g / L , pH7.3; anaerobic tube slant medium needs to add agar, the concentration of agar is 20g / L. After deoxygenation, it was sterilized by 0.1MPa steam for 20 minutes for later use.

[0024] (2) Seed expansion culture and collection of bottled seed cells

[0025] According to the requirements of aseptic and anaerobic operation, the cells were transferred from the freeze-dried tube of Butyricum strain to the newly prepared slant medium of anaerobic tube. After culturing at 37°C for 12 hours, according to the requirements of sterile and anaerobic operation, add 10 mL of sterile and anaerobic water to prepare anaerobic tube seed solution.

[0026] 120mL anaerobic bottle culture medium 60mL, put the butyric acid bacteria anaerobic tube seed s...

Embodiment 2

[0032] (1) Preparation of medium: same as in Example 1.

[0033] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: same as in Example 1.

[0034] (3) Fermentation culture: Except for exponential feeding, the remaining conditions of fermentation culture are the same as in Example 1.

[0035] Exponential feeding: Fermentation starts at 6h, and the specific growth rate is set at 0.112h -1 (20% of the maximum specific growth rate), add glucose solution (concentration: 500g / L) to the fermentation culture for 13h.

[0036] The exponential flow addition equation is F=μ(VX) 0 exp(μt) / Y x / s (S F -S),

[0037] In the formula, F, X and S are flow acceleration rate, cell and substrate concentration (g / L), respectively, and μ is specific growth rate (h -1 ), V is the fermentation broth volume (L), S F is the concentration of added glucose (g / L), Y x / s is the yield coefficient (g / g) of the cell to the substrate (glucose), (VX) 0 is the initial cell m...

Embodiment 3

[0044] (1) Preparation of medium: same as in Example 1.

[0045] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: same as in Example 1.

[0046] (3) Fermentation culture: Except for exponential feeding, the remaining conditions of fermentation culture are the same as in Example 1.

[0047] Exponential feeding: Fermentation starts at 6h, and the specific growth rate is set at 0.35h -1 (62% of the maximum specific growth rate), a glucose solution (concentration of 500 g / L) was added to the fermentation culture for 13 hours. Calculate the glucose solution flow acceleration rate setting value according to the exponential feed-adding equation in embodiment 2, as shown in table 2:

[0048] Table 2

[0049] Fermentation time (h) Flow acceleration rate (mL / h) Mass rate of glucose feeding (g / h) 6-7 370 185 7-8 520 260 8-9 740 370 9-10 1050 525 10-11 1490 745 11-12 2120 1060 12-13 3000 1500

...

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Abstract

The invention discloses a feeding method for improving the fermentation growth efficiency of butyric acid bacteria, and belongs to the technical field of agricultural living things. The feeding method for improving the fermentation growth efficiency of butyric acid bacteria is realized through optimizing the specific growth rate of the butyric acid bacteria and controlling acid metabolism, and is concretely characterized in that exponential fed-batch of a glucose solution is carried out from the 6th hour to the 13th hour of the butyric acid bacterium fermentation culture process at a specific growth rate of 0.1-0.4 h<-1> and preferably 0.225 h<-1>. The method has the characteristics of simple and clear fed components, low cost, clear fed0batch rate, high operating controllability, and suitableness for fermenting production. The method overcomes the technical prejudice that the preset value of the specific growth rate of exponential fed-batch approaches 80-90% of a maximum specific growth rate, and has the advantages of great promotion of the logarithmic growth of butyric acid bacterium cells through exponential fed-batch at a low preset value, substantial reduction of the concentrations of acetic acid and butyric acid generated in the fermentation process, and extremely technical effects.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a feeding method for improving the fermentation growth efficiency of butyric acid bacteria. Background technique [0002] Butyric acid bacteria (Clostridium butyrium), as a new probiotic for humans or animals, has important functions such as adjusting the balance of intestinal flora, enhancing immunity, and preventing tumorigenesis. The nutritional requirements of butyric acid bacteria have a significant impact on their growth. The commonly used feeding method is to add nitrogen sources such as peptone, yeast extract, soybean meal, soybean flour hydrolyzate, fish meal, etc., and carbon sources such as glucose, etc., such as: Reference 1 (using A method for preparing live butyric acid bacteria preparations by fed-batch fermentation, invention patent CN201110287802.1, 2011) discloses adding 10-20% glucose solution to 12-14 hours of fermentation of butyric a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145
CPCC12N1/20C12N1/205C12R2001/145
Inventor 王志夏会丽陈雄李冬生
Owner HUBEI UNIV OF TECH
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