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Escherichia coli and/or salmonella and/or listeria monocytogenes enzyme production co-culture medium

A technology for Listeria monocytogenes and Salmonella, which is applied in the biological field, can solve the problems that the detection results are not very reliable, Salmonella cannot produce octyl esterase well, etc., and achieve remarkable results, ensure reliability, and improve detection speed. Effect

Inactive Publication Date: 2017-04-26
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practice, the Salmonella pre-enriched in this medium cannot produce octyl esterase well. When using a disc containing β-naphthol octanoate to detect the enrichment solution containing this Escherichia coli and Salmonella, There is no specific color change in the Salmonella detection part, and the detection result is not very reliable

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0007] Embodiment 1, a kind of Escherichia coli and Salmonella enzyme-producing co-culture medium, this medium contains caseinase digestate 17g, yeast extract 6g, glucose 2.5g, soybean peptone 3g, sodium chloride 5g, phosphoric acid per liter of sterile distilled water Potassium dihydrogen 1.35g, dipotassium hydrogen phosphate 2.5g, disodium hydrogen phosphate 9.6g, and sodium pyruvate 1.1g; the above-mentioned substances were dissolved in sterile distilled water, sterilized under high pressure at 121°C for 15min, cooled to room temperature, and stored in cold storage. Get the culture medium. In practice, the samples to be tested were inoculated with the above medium, incubated at 37°C for 4 hours, and then 0.05 g / L cycloheximide and 0.05 g / L fosfomycin were added; the addition of antibiotics can well inhibit the growth of miscellaneous bacteria and significantly promote Production of Escherichia coli β-D-galactosidase and Salmonella octanoesterase. Salmonella can produce oct...

Embodiment 2

[0008] Embodiment 2, a kind of escherichia coli and Salmonella and Listeria monocytogenes enzyme-producing co-culture medium, this culture medium comprises caseinase digestate 17g, yeast extract 6g, glucose 2.5g, soybean peptone 3g, Sodium chloride 5g, potassium dihydrogen phosphate 1.35g, dipotassium hydrogen phosphate 2.5g, disodium hydrogen phosphate 9.6g and sodium pyruvate 1.1g; the above-mentioned substances were dissolved in sterile distilled water, sterilized under high pressure at 121°C for 15min, and cooled To room temperature, refrigerated to prepare the culture medium; then inoculate the test sample with the above medium, and culture at 37°C for 4 hours; provide all the nutritional requirements of Escherichia coli, Salmonella and Listeria monocytogenes, and then add cycloheximide 0.05 g / L and fosfomycin 0.05 g / L, significantly inhibited the growth of miscellaneous bacteria, at the same time, the amount of octyl esterase produced by Salmonella significantly increased...

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly discloses an escherichia coli and / or salmonella and / or listeria monocytogenes enzyme production co-culture medium. The culture medium comprises 17 g of casease digesta, 6 g of yeast paste, 2.5 g of glucose, 3 g of soytone, 5 g of sodium chloride, 1.35 g of monopotassium phosphate, 2.5 g of dipotassium phosphate, 9.6 g of disodium phosphate and 1.1 g of sodium pyruvate in one liter of sterile distilled water. The substances are dissolved into the sterile distilled water, sterilized for 15 min at high pressure at the temperature of 121 DEG C, cooled to the room temperature and stored in a refrigeration mode to prepare the culture medium. In the practice, the detection speed is greatly increased, reliability of detection results is ensured, and the effect is remarkable.

Description

technical field [0001] The invention belongs to the field of biotechnology and specifically discloses an enzyme-producing co-culture medium for Escherichia coli and / or Salmonella and / or Listeria monocytogenes. The culture medium can be used for single or simultaneous cultivation of Escherichia coli, Salmonella, and Listeria monocytogenes, and is used for the pre-enrichment of bacteria in the detection method based on bacterial specific enzymes. Background technique [0002] In order to realize the rapid detection of Escherichia coli, Salmonella and Listeria monocytogenes that may exist in food, water sources, milk and beverages, etc., it is necessary to realize the co-cultivation of the picked target bacteria, save the cultivation time through co-cultivation, and truly achieve rapid detection the goal of. Therefore, finding a suitable co-culture medium for the above two or three bacteria becomes the key to rapid qualitative detection. The compound medium (SEL) of Salmonell...

Claims

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Application Information

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IPC IPC(8): C12Q1/44C12Q1/34C12Q1/10C12Q1/04C12R1/19C12R1/42C12R1/01
Inventor 夏利宁姚刚帕力达·亚森苏战强刘英玉钟旗郑小凤张琦张晓红
Owner XINJIANG AGRI UNIV
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