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Biological hydrogel for 3D printed tissue engineering scaffold and preparation method of biological hydrogel

A tissue engineering scaffold and 3D printing technology, applied in the field of 3D printing, can solve the problems of poor mechanical properties of collagen, fast degradation speed, scaffold collapse, etc., and achieve the effect of increasing added value, good biodegradability and biocompatibility

Inactive Publication Date: 2017-05-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of pure collagen are poor, and the degradation rate is too fast under the action of various enzymes in the body, and the scaffold has collapsed before the new tissue grows well.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] (1) The mass percentage of raw materials used: DANCC 50%, COL (Type I) 50%;

[0022] (2) The solvent used is 0.5mol / L acetic acid aqueous solution, and the mass ratio of the total mass of solute to the solvent is 2:98;

[0023] (3) DANCC and COL were blended at 4°C for 6 hours, and the stirring speed was 1000rpm;

[0024] (4) The crosslinking temperature of DANCC and COL blend is 15°C, and the crosslinking time is 24h;

[0025] (5) Aseptically store at 1°C for later use.

specific Embodiment approach 2

[0027] (1) The mass percentage of raw materials used: DANCC40%, COL (Type I, Type I) 40%, CS20%;

[0028] (2) The solvent used is a mixed solution of hexafluoroisopropanol and water, and the mass ratio of the total mass of the solute to the solvent is 4:96;

[0029] (3) Blend DANCC, COL and CS at 2°C for 16 hours, stirring at 800 rpm;

[0030] (4) The crosslinking temperature of DANCC and COL blend is 19°C, and the crosslinking time is 16h;

[0031] (5) Aseptically store at 3°C ​​for later use.

specific Embodiment approach 3

[0033] (1) The mass percentage of raw materials used: DANCC30%, COL (Type I, Type I) 40%, CS30%;

[0034] (2) The solvent used is a mixed solution of trifluoroethanol and water, and the mass ratio of the total mass of the solute to the solvent is 5:95;

[0035] (3) DANCC, COL and CS were blended at 8°C for 8 hours, and the stirring speed was 800rpm;

[0036] (4) The crosslinking temperature of DANCC and COL blend is 25°C, and the crosslinking time is 20h;

[0037] (5) Aseptically store at 5°C for later use.

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Abstract

The invention relates to biological hydrogel for a 3D printed tissue engineering scaffold and a preparation method of the biological hydrogel and belongs to the technical field of 3D printing. The biological hydrogel is prepared from three or any two of raw materials in percentage by mass as follows: 20%-70% of DANCC (double aldehyde nano-crystalline cellulose), 20%-70% of COL (collagen) (types I and II) and 20%-60% of CS (chitosan). DANCC serves as a crosslinking agent to modify COL and / or CS, molecules of COL and CS can interact, two modification methods are combined, the stability and mechanical properties of the biological hydrogel are better improved, and biocompatibility and biodegradability are also improved. Results indicate that the anti-compression breaking strength of the hydrogel is improved by 8-15 times after addition of DANCC.

Description

technical field [0001] The invention belongs to the technical field of 3D printing, and relates to a biohydrogel for 3D printing tissue engineering scaffolds and a preparation method thereof, in particular to a biohydrogel having biocompatibility, biodegradability, and bioactivity and satisfying Composite material required for tissue engineering scaffold and preparation method thereof. Background technique [0002] Tissue defect repair and organ transplantation are one of the greatest breakthroughs in the history of medicine in the 20th century. At present, there are hundreds of millions of patients in need of tissue defect repair in the world, and nearly 1 million patients have been reborn through organ transplantation, but tens of millions of patients have died due to lack of donor organs. The emergence of bio-3D printing technology has brought a new dawn to tens of millions of patients who need organ transplantation and tissue repair. [0003] The existing 3D printing t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/50A61L27/20A61L27/24
CPCA61L27/52A61L27/26A61L27/50A61L2430/40B33Y70/00C08J3/24C08J2305/08C08J2389/00C08J2405/08C08J2489/00C08L1/08C08L89/00C08L5/08
Inventor 周骥平姜亚妮许晓东张琦朱兴龙赵国琦
Owner YANGZHOU UNIV