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A kind of nattokinase microcapsule and preparation method thereof

A technology of soykinase microcapsules and nattokinase, which is applied to microcapsules, biochemical equipment and methods, capsule transportation and other directions, can solve the problems of inability to directly obtain microcapsule powder, complicated steps, etc., and achieves stable enzyme activity and simple process. , the effect of improving safety

Active Publication Date: 2021-01-05
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods all prepare the liquid core of the microcapsules by electrostatic spraying, and then use the wall material for reinforcement. The follow-up steps are cumbersome and need to go through processes such as filtration, cleaning, and drying, and the microcapsule powder cannot be obtained directly.

Method used

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  • A kind of nattokinase microcapsule and preparation method thereof

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Effect test

Embodiment 1

[0030] A preparation method for nattokinase microcapsules, comprising the steps of:

[0031] 1) Selection of raw materials: according to the ratio of whey protein, nattokinase and deionized water as 12g:1g:100mL, weigh whey protein, nattokinase and solvent deionized water for later use;

[0032] 2) Preparation of emulsion: According to the proportioning of raw materials weighed in step 1), whey protein was dissolved in deionized water to prepare a 12% aqueous solution, stirred overnight at room temperature, then added nattokinase and stirred evenly, 6000r Homogenize for 6 minutes under the condition of / min to obtain the emulsion;

[0033] 3) Emulsion electrospray: The emulsion obtained in step 2) is electrosprayed with an electrostatic spray device, and aluminum foil is used as the receiver. The electrospray conditions are: needle head 18G, voltage 13KV, emulsion flow rate 1ml / h, needle tip to accept aluminum foil The distance is 10cm; the solid powder obtained after electro...

Embodiment 2

[0036] A preparation method for nattokinase microcapsules, comprising the steps of:

[0037] 1) Selection of raw materials: according to the ratio of zein, nattokinase and 75wt% ethanol water as 10g: 2g: 100mL, weigh zein, nattokinase and solvent deionized water for later use;

[0038] 2) Preparation of emulsion: According to the proportioning of raw materials weighed in step 1), zein is dissolved in 75wt% ethanol water, and is prepared into a 10% aqueous solution, stirred overnight at room temperature, then added nattokinase and stirred evenly , homogenized for 8 minutes under the condition of 5000r / min to obtain the emulsion;

[0039] 3) Emulsion electrospray: The emulsion obtained in step 2) is electrosprayed with an electrostatic spray device, and aluminum foil is used as the receiver. The electrospray conditions are: needle 20G, voltage 10KV, emulsion flow rate 0.5ml / h, needle point to accept The distance of the aluminum foil is 8cm; the solid powder obtained after elect...

Embodiment 3

[0042] A preparation method for nattokinase microcapsules, comprising the steps of:

[0043] 1) Selection of raw materials: according to the ratio of whey protein, nattokinase and deionized water is 15g: 1.5g: 100mL, weigh whey protein, nattokinase and solvent deionized water for later use;

[0044] 2) Preparation of emulsion: according to the proportioning of the raw materials weighed in step 1), whey protein was dissolved in deionized water, and prepared into a 15% aqueous solution, stirred overnight at room temperature, then added nattokinase and stirred evenly, 10000r Homogenize for 3 minutes under the condition of / min to obtain the emulsion;

[0045]3) Emulsion electrospray: The emulsion obtained in step 2) is electrosprayed with an electrostatic spray device, and aluminum foil is used as the receiver. The electrospray conditions are: needle 21G, voltage 15KV, emulsion flow rate 1ml / h, needle tip to accept aluminum foil The distance is 12cm; the solid powder obtained af...

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Abstract

The invention discloses a natto kinase microcapsule. The natto kinase microcapsule uses proteins as the wall material of the microcapsule and natto kinase as the core material of the microcapsule, and is a regular circular particle of which the average particle diameter is 300-800nm; the natto kinase microcapsule has no viscosity; and the activity retention rate is 45% or above. The preparation method comprises the following steps: dissolving the wall material and core material in a solvent, uniformly mixing, and carrying out electronic injection on the obtained emulsion under the action of an electrostatic field to obtain solid powder which is the natto kinase microcapsule. The natto kinase microcapsule has the function of protecting the natto kinase, and enhances the stability of the natto kinase; particularly, the enzyme activity at 4 DEG C and 37 DEG C is stabler than that of the free natto kinase; the natto kinase microcapsule is prepared by carrying out microencapsulation at 25-40 DEG C, thereby avoiding the influence of spray drying on activity and effectively controlling the particle size of the microcapsule; and the natto kinase microcapsule has the advantages of high activity retention rate and smaller particle size.

Description

technical field [0001] The invention relates to a nattokinase microcapsule and a preparation method thereof, belonging to the technical field of microcapsules. Background technique [0002] Nattokinase is a Bacillus subtilis enzyme extracted from natto, a traditional Japanese food, which has a highly effective antithrombotic effect. Compared with other traditional thrombolytic agents, nattokinase has the advantages of good safety, low cost, easy absorption by the human body, direct and rapid action and long duration, and has become a new generation of thrombolytic agent with great potential. Temperature and acidic conditions will affect the activity of nattokinase. The enzyme activity of nattokinase is relatively stable below 37°C, and the enzyme activity is relatively stable between pH 5 and 11. At present, the thrombolytic agent of nattokinase is mostly taken in the form of hard capsules, but the traditional hard capsules have affected the absorption and utilization of na...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61K9/50A61K47/42A61P7/02
CPCA61K9/5052A61K9/5057A61K9/5089A61K38/482C12Y304/21062
Inventor 董绪燕幸红林杜姗姗吴邦富魏芳陈洪吕昕黄凤洪邓乾春
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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