Double-layer silk fibroin film, preparation method and application

Abstract

The invention relates to a preparation method of a double-layer silk fibroin film. The preparation method comprises preparation of a silk fibroin evaporated film and preparation of an electrostatic spinning film, wherein the electrostatic spinning film is prepared as follows: 5 ml of a water solution with the concentration of 3% is prepared from silk fibroin powder; the solution is put in an injector, and the electrostatic spinning film is prepared on the surface of the evaporated film by use of a NO.7 needle and existing electrostatic spinning equipment; parameters of instruments are set as follows: the loading voltage is 10-20 kV, the distance between the injector and a receiving plate is 7-12 cm, the pushing speed is 0.1-0.3 ml / h, and the relative humidity of air is 35%-45%; and the double-layer film can be obtained on the surface of the evaporated film after electrostatic spinning is completed. Bionic structures of an inner layer and an outer layer of the double-layer bone-like film play own roles respectively, and the double-layer silk fibroin film has the result similar to that of autogenous bone; silk fibroin has better biocompatibility; the double-layer film is prepared with solvent evaporation and electrostatic spinning technologies, can better simulate the micro-nano fiber network structure of a bone film and better facilitates growth and infiltration of bone cells, thereby favorable for bone regeneration and repairing.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a silk fibroin double-layer film, a preparation method and application thereof, wherein a fibrous film with a double-layer structure is prepared by using the silk fibroin. Background technique [0002] The periosteum is composed of two parts. The outer layer is mainly composed of collagen fibers tightly combined and rich in blood vessels and nerves; the inner layer is also called the cambium, which is composed of thicker collagen fibers, osteoblasts, endothelial cells, and stem cells. At present, the research and application of periosteum-like artificial repair materials are attracting more and more attention. These materials can be divided into degradable (such as polylactic acid, collagen membrane) and non-degradable (such as polytetrafluoroethylene membrane), and their functions are: Provide space for bone regeneration; prevent surrounding connective tissue from growing into the def...

AI Technical Summary

Problems solved by technology

Non-degradable materials are easy to cause infection due to long-term retention, so secondary surgery is required to remove them; while degradable materials do not require secondary surgery to remove, but their degradation products will be retained locally, which can easily lead to inflammatory reactions

(2) Poor mechanical properties

Non-degradable materials are hard and difficult to cut, shape, and sew; degradable materials have poor mechanical properties and poor maintenance of regeneration space

(3) No vascularization function

These materials give more consideration to their interaction with bone-forming cells, but without the function of natural periosteum to supply blood to bone tissue, it is still difficult to achieve rapid repair of bone defects

Therefore, it is still challenging to develop a bilayer repair material that is closer to the natural periosteum.