Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for colon adenocarcinoma

A technology of colon adenocarcinoma and primer pairs, applied in the field of biomedicine, to improve the quality of life, reduce the cost of treatment, and improve the quality of life

Active Publication Date: 2017-05-17
CHINA JAPAN FRIENDSHIP HOSPITAL
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on lncRNA is still in its infancy, and the understanding of its mechanism and function is still being explored

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for colon adenocarcinoma
  • Molecular marker for colon adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Screening for Gene Markers Related to Colon Adenocarcinoma

[0052] 1. Collection of samples

[0053] Eight cases of colon adenocarcinoma tissue and paracancerous mucosal tissue samples were collected. All patients had not received radiotherapy or chemotherapy before surgery, and all patients gave informed consent. All the above samples were obtained with the consent of the organizational ethics committee.

[0054] 2. Preparation of RNA samples (operated using QIAGEN tissue RNA extraction kit)

[0055] 1) Make ice, take the tissue and put it into a mortar, grind it into fine powder, keep the tissue frozen during the grinding process.

[0056] 2) Transfer the tissue into a 1.5ml EP tube, add 1ml Trizol reagent, grind finely with a grinding rod, fully homogenize with a vortex mixer, and ice-bath for 10 minutes.

[0057] 3) Centrifuge at 12000 rpm for 10 min at 4°C.

[0058] 4) Pipette the upper aqueous phase into another new 1.5ml EP tube, add isopropanol equ...

Embodiment 2

[0074] Example 2 QPCR sequencing to verify the differential expression of the LOC105377352 gene

[0075]1. According to the detection results of high-throughput sequencing, the LOC105377352 gene was selected for large-sample QPCR verification. According to the sample collection method in Example 1, 60 cases of colon adenocarcinoma tissues and 60 cases of colon adenocarcinoma paracancerous mucosa tissues were selected.

[0076] 2. The RNA extraction steps are the same as in Example 1.

[0077] 3. Reverse transcription:

[0078] 1) Reaction system:

[0079] Reagent volume MgCl 2

2μl 10×RT Buffer 1μl RNase-free water 3.75μl dNTP mix 1μl RNase inhibitor 0.25μl AMV reverse transcriptase 0.5μl Oligo-dT Adapter Primer 0.5μl Experimental sample 1μl

[0080] 2) Reverse transcription reaction conditions

[0081] According to the reverse transcription reaction conditions in RNA PCR Kit (AMV) Ver.3.0.

[0082...

Embodiment 3

[0100] Embodiment 3 Preparation of early colon adenocarcinoma diagnostic kit

[0101] According to the correlation between the LOC105377352 gene and colon adenocarcinoma, colon adenocarcinoma can be diagnosed by detecting the expression of the LOC105377352 gene. Accordingly, the present invention provides a kit for diagnosing colon adenocarcinoma based on detecting the expression of the LOC105377352 gene. The diagnostic reagent The components in the box are as follows: SYBR Green polymerase chain reaction system; primer pair for amplifying LOC105377352 gene and GAPDH gene. The primer pair sequences for amplifying the LOC105377352 gene are shown in SEQ ID NO.2 and SEQ ID NO.3; the primer pair sequences for amplifying GAPDH are shown in SEQ ID NO.4 and SEQ ID NO.5.

[0102] The SYBR Green polymerase chain reaction system includes PCR buffer, dNTPs, and SYBR Green fluorescent dye. PCR buffer composition: 25mM KCl, 2.5mM MgCl 2 , 200mM (NH4) 2 SO 4 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker for colon adenocarcinoma. The molecular marker is LOC105377352, wherein the expression of the LOC105377352 in a patient of the colon adenocarcinoma is increased. The lncRNA (long non-coding Ribonucleic Acid) marker provided by the invention can be used for early diagnosis of the colon adenocarcinoma and has relatively high sensitivity and specificity.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a molecular marker for colon adenocarcinoma, in particular to a molecular marker LOC105377352 for early colon adenocarcinoma. Background technique [0002] Colon cancer is one of the most common malignant tumors of the digestive tract in my country, and its incidence rate ranks third in my country, second only to lung cancer and gastric cancer, seriously endangering people's health. In recent years, with the continuous improvement of people's living standards, changes in eating habits and dietary structure, and population aging, the incidence and mortality of colon cancer in my country have maintained an upward trend. The occurrence of colon cancer is affected by genetic and internal and external environmental factors, and its pathogenesis is a comprehensive and complex pathological process involving multiple factors, multiple steps, and multiple stages, including intestinal epithelial ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/158C12Q2600/178G01N33/57419
Inventor 刘继喜刘芳谭煌英
Owner CHINA JAPAN FRIENDSHIP HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com