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Antibacterial artificial dermal scaffold and preparation method of antibacterial artificial dermal scaffold

A dermal and artificial technology, applied in the medical field, can solve the problems affecting the success rate of product implantation, poor anti-infection effect, poor anti-scar effect, etc., so that the operation process can be monitored in real time, wound healing can be promoted, and the biological phase can be improved. Capacitive and degradable effects

Inactive Publication Date: 2017-05-24
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After implantation, the interface between the artificial skin and the wound is prone to infection. Whether this shortcoming can be overcome will seriously affect the success rate of product implantation.
The existing artificial dermal scaffolds all have the risk of wound infection during wound repair, and some artificial dermal scaffolds have disadvantages such as poor anti-infection effect and poor anti-scar effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 0.941 g of collagen and dissolve it in 100 mL of 0.15 mol / L acetic acid solution. Weigh 0.014 g lysostaphin and 0.126 g lysozyme and dissolve in 100 mL 0.15 mol / L acetic acid solution. Weigh 0.120 g chondroitin sulfate and dissolve it in 100 mL 0.15 mol / L acetic acid solution.

[0025] The biological enzyme solution and chondroitin sulfate solution were added dropwise to the collagen solution at a rate of 998 μL / min, and stirred continuously to obtain a collagen complex with a concentration of 0.4%.

[0026] The obtained collagen composite was poured into a polytetrafluoroethylene mold, frozen at -80°C for 1.5 hours, and then placed in a freeze dryer for 24 hours to freeze-dry to obtain a collagen composite sponge scaffold with a three-dimensional porous structure.

[0027] The obtained collagen composite sponge scaffold is immersed in a glutaraldehyde-acetic acid solution, the volume fraction of glutaraldehyde in the glutaraldehyde-acetic acid solution is 0.2%, and the...

Embodiment 2

[0030] Weigh 1.482 g of collagen and dissolve it in 100 mL of 0.15 mol / L acetic acid solution. Weigh 0.019 g of lysostaphin and 0.170 g of lysozyme in 100 mL of 0.15 mol / L acetic acid solution. Weigh 0.176 g hyaluronic acid and dissolve it in 100 mL 0.15 mol / L acetic acid solution.

[0031] The biological enzyme solution and the hyaluronic acid solution were added dropwise to the collagen solution at a rate of 998 μL / min, and continuously stirred to obtain a collagen complex with a concentration of 0.6%.

[0032] The obtained collagen composite was poured into a polytetrafluoroethylene mold, frozen at -80°C for 1.5 hours, and then placed in a freeze dryer for 24 hours to freeze-dry to obtain a collagen composite sponge scaffold with a three-dimensional porous structure.

[0033] The obtained collagen composite sponge scaffold is immersed in a glutaraldehyde-acetic acid solution, the volume fraction of glutaraldehyde in the glutaraldehyde-acetic acid solution is 0.1%, and the concent...

Embodiment 3

[0035] Weigh 0.980 g of collagen and dissolve it in 100 mL of 0.15 mol / L acetic acid solution. Weigh 0.028 g of lysostaphin and 0.250 g of lysozyme in 100 mL of 0.15 mol / L acetic acid solution. Weigh 0.235 g of chitosan and dissolve it in 100 mL of 0.15mol / L acetic acid solution.

[0036] The biological enzyme and chondroitin sulfate solution were added dropwise to the collagen solution at a rate of 998 μL / min, and continuously stirred to obtain a collagen complex with a concentration of 0.8%.

[0037] The obtained collagen composite was poured into a polytetrafluoroethylene mold, frozen at -60°C for 3 hours, and then placed in a freeze dryer for freeze drying for 24 hours to obtain a collagen composite sponge scaffold with a three-dimensional porous structure.

[0038] The obtained collagen composite sponge scaffold is immersed in a glutaraldehyde-acetic acid solution, the volume fraction of glutaraldehyde in the glutaraldehyde-acetic acid solution is 0.25%, and the concentration o...

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Abstract

The invention discloses an antibacterial artificial dermal scaffold and a preparation method of the antibacterial artificial dermal scaffold. The antibacterial artificial dermal scaffold is prepared from collagen, a biological enzyme and a third material, wherein the third material is selected from at least one of chondroitin sulfate, hyaluronic acid, chitosan and silk fibroin. Lysozyme is added into a collagen protein scaffold of the artificial dermal scaffold, so that the obtained artificial dermal scaffold has functions of resisting bacteria, resisting infection and accelerating wound healing; the antibacterial artificial dermal scaffold prepared by the invention has very good biocompatibility and degradability, has suitable softness and can be fitted with a wound very well; the preparation method of the antibacterial artificial dermal scaffold is simple and an operation process can be monitored in real time.

Description

Technical field [0001] The invention relates to the field of medical technology, in particular to an antibacterial artificial dermal stent and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body. It protects the body from harmful substances from the outside, prevents the loss of water and electrolytes in the body, maintains the stability of the human body environment, and participates in the body's immune and metabolic processes. In our country, nearly 10 million people need skin transplantation due to burns and skin ulcers each year. Skin defects and necrosis caused by various reasons will directly affect people's quality of life and even threaten lives. Although the skin can regenerate and repair on its own, it is limited to superficial damage. When the skin is severely burned and damaged (deep II degree and III degree), the skin cannot repair itself, and skin graft surgery is required to complete the wound repair. [0003...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/26A61L27/54A61L27/56A61L27/58
CPCA61L27/60A61L27/26A61L27/54A61L27/56A61L27/58A61L2300/254A61L2300/404C08L5/08
Inventor 佘振安
Owner SHENZHEN QIKANG MEDICAL DEVICES
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