Strain of pseudomonas poae and application thereof to sewage treatment

A technology for sewage treatment and Pseudomonas, applied in biological water/sewage treatment, water/sludge/sewage treatment, fixed on/in organic carriers, etc., can solve the problem of difficult long-term operation and competition of indigenous bacteria Disadvantages, cell loss and other issues, to achieve the effects of low cost, guaranteed microbial biomass and cell activity, and wide application

Inactive Publication Date: 2017-05-24
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The screening and application research of psychrotrophic bacteria in sewage treatment can improve the efficiency of sewage treatment, but when directly added

Method used

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  • Strain of pseudomonas poae and application thereof to sewage treatment
  • Strain of pseudomonas poae and application thereof to sewage treatment
  • Strain of pseudomonas poae and application thereof to sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1 Test materials and methods

[0029] 1.1 Source of bacteria

[0030] Activated sludge from SBR reaction tank of Chongli Sewage Treatment Plant in Zhangjiakou City, Hebei Province.

[0031] 1.2 Enrichment and separation medium

[0032] Cold-resistant bacteria culture medium: CH 3 COONa, 3.0 g; KNO 3 , 1.0 g; K 2 HPO 4 , 0.1 g; KH 2 PO 4 , 0.1 g; NH 4 Cl, 0.2g; MgSO 4 ·7H 2 O, 0.6 g; CaCl 2 ·2H 2 O, 0.07 g; trace element solution, 2 ml; 1 L of distilled water.

[0033] Trace element: FeCl 3 ·6H 2 O, 1.5 g; CuSO 4 ·5H 2 O, 0.03 g; MnCl 2 ·4H 2 O, 0.12 g; ZnSO 4 ·7H 2 O, 0.12 g; Na 2 MoO 4 ·2H 2 O, 0.06 g; H 3 BO 3 , 0.15 g; KI, 0.03 g; CoCl 2 ·6H 2 O, 0.15g; Distilled water, 1 L.

[0034] The culture medium was sterilized in a pressure cooker at 121 ℃ for 30 min before use, adjusted to pH = 7.0 ~ 7.2, the operation is aseptic. The solid medium is added with 2% (W / V) agar powder on the basis of the liquid medium.

[0035] 1.3 Screening of cold-tolerant bacteria

[0036] Take 10 mL of ...

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PUM

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Abstract

The invention discloses a strain of pseudomonas poae and application thereof to sewage treatment, and relates to the technical field of environmental microorganism and sewage treatment. The pseudomonas poae is named as NL-4; the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.13450. Polyvinyl alcohol and sodium alginate are used for embedding the pseudomonas poae NL-4, and an immobilized carrier of the pseudomonas poae NL-4 can be obtained. The NL-4 and the immobilized carrier can be applied to the sewage treatment. The pseudomonas poae can improve the sewage biologic treatment efficiency under the low-temperature condition; an effective removal effect is achieved on NH3-N, NO3<->-N and COD in sewage; the outlet water standard reaching rate is improved by enriching the quantity and improving the activity of cold-resistant microorganisms in sewage biological treatment buildings. The method has the advantages that the operation is simple; the cost is low; economic performance and environment protection are realized; the pseudomonas poae can be singly used or can be applied to wastewater treatment after the immobilization; the application is wide.

Description

Technical field [0001] The invention relates to the technical field of environmental microorganisms and sewage treatment. Background technique [0002] Biological treatment is the most commonly used and most cost-effective technical means for sewage treatment. The key to achieving pollutant discharge is that there are sufficient microorganisms in the biological treatment structure that have the ability to decompose pollutants. Biological treatment technology has the problems of poor treatment effect and difficulty in meeting discharge standards when the temperature is low in winter in cold regions. Sewage treatment plants generally reduce sludge load and increase sludge concentration to ensure the quality of effluent water, but at the same time it will increase operating costs. Sudarno (Effect of varying salinity, temperature, ammonia and nitrous acid concentrations on nitrification of saline wastewater in fixed-bed reactors.Bioresour Technol, 2011,102(10):5665-5673) and Kroupov...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12N11/10C12R1/38C02F101/38
CPCC02F3/34C02F2101/38C12N11/10C12N1/205C12R2001/38
Inventor 宋漫利梁致齐刘春敬谢建治李荣榛
Owner HEBEI AGRICULTURAL UNIV.
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