Strain of pseudomonas poae and application thereof to sewage treatment
A technology for sewage treatment and Pseudomonas, applied in biological water/sewage treatment, water/sludge/sewage treatment, fixed on/in organic carriers, etc., can solve the problem of difficult long-term operation and competition of indigenous bacteria Disadvantages, cell loss and other issues, to achieve the effects of low cost, guaranteed microbial biomass and cell activity, and wide application
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[0028] 1 Test materials and methods
[0029] 1.1 Source of bacteria
[0030] Activated sludge from SBR reaction tank of Chongli Sewage Treatment Plant in Zhangjiakou City, Hebei Province.
[0031] 1.2 Enrichment and separation medium
[0032] Cold-resistant bacteria culture medium: CH 3 COONa, 3.0 g; KNO 3 , 1.0 g; K 2 HPO 4 , 0.1 g; KH 2 PO 4 , 0.1 g; NH 4 Cl, 0.2g; MgSO 4 ·7H 2 O, 0.6 g; CaCl 2 ·2H 2 O, 0.07 g; trace element solution, 2 ml; 1 L of distilled water.
[0033] Trace element: FeCl 3 ·6H 2 O, 1.5 g; CuSO 4 ·5H 2 O, 0.03 g; MnCl 2 ·4H 2 O, 0.12 g; ZnSO 4 ·7H 2 O, 0.12 g; Na 2 MoO 4 ·2H 2 O, 0.06 g; H 3 BO 3 , 0.15 g; KI, 0.03 g; CoCl 2 ·6H 2 O, 0.15g; Distilled water, 1 L.
[0034] The culture medium was sterilized in a pressure cooker at 121 ℃ for 30 min before use, adjusted to pH = 7.0 ~ 7.2, the operation is aseptic. The solid medium is added with 2% (W / V) agar powder on the basis of the liquid medium.
[0035] 1.3 Screening of cold-tolerant bacteria
[0036] Take 10 mL of ...
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