Method for colonizing Beauveria bassiana in corn seedling stage and use thereof

A technology for Beauveria bassiana and corn seedlings, applied in the field of microbial colonization, can solve problems such as changes in defense enzyme activity, and achieve the effects of enhanced resistance, protection from pest infestation, and simple and easy colonization methods

Inactive Publication Date: 2017-05-31
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports about the mechanism of entomogenic fungi promoting plant growth. Changes in the growth status of plants will inevitably lead to changes in the activities of related defense enzymes in plants. There are no reports on this aspect so far.

Method used

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  • Method for colonizing Beauveria bassiana in corn seedling stage and use thereof
  • Method for colonizing Beauveria bassiana in corn seedling stage and use thereof
  • Method for colonizing Beauveria bassiana in corn seedling stage and use thereof

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Embodiment 1

[0034] This embodiment provides a method for colonizing Beauveria bassiana at the seedling stage of corn, including:

[0035] The present invention adopts random block design, potted plant control experiment. Two levels were set for soaking time and Beauveria bassiana treatment. The experiment set up 4 treatments, 8 corn plants in each treatment, and 10 repetitions. The experimental treatments were soaking seeds in Tween-80 for 12 hours (T80-12h), soaking seeds in Beauveria bassiana spore suspension for 12 hours (Bb-12h), soaking seeds in Tween-80 for 24 hours (T80-24h), soaking seeds in Beauveria bassiana spore suspension Soak the seeds in the suspension for 24h (Bb-24h).

[0036] Select corn seeds with uniform particle size, and disinfect the surface of the seeds (first soak in 70% ethanol solution for 30s, then soak in 1% sodium hypochlorite solution for 30s, and finally wash with sterile water for 3 times), every 100 corn seeds (about 23g) Soak in 20mL Beauveria bassian...

Embodiment 2

[0038] This embodiment provides the detection method and result of the effect of Embodiment 1.

[0039] 1. Experimental method

[0040] 1. Investigation on the colonization preference of Beauveria bassiana

[0041] The sampling period is 3 times, respectively 14d, 21d, and 28d for the emergence of corn in Example 1. Each period is treated with 10 seedlings, and each seedling is taken with 9 pieces of roots, stems and leaves. The length of the rhizome is 1 cm, and the area of ​​the leaves is 1 cm . Colonization rate detection adopts PDA detection method, sample material surface disinfection (seed disinfection method in embodiment 1), every kind of tissue is placed in a PDA selection culture dish (9 samples of each plate), 28 ℃, in the incubator Dark cultivation. After 7 days, observe and record the growth of the bacteria on the plant tissue every day. The colonization rate = the number of plates with colonies in the plant tissue / the total number of plates × 100, and the aver...

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Abstract

The invention relates to the technical field of microbial colonization and concretely relates to a method for colonizing Beauveria bassiana in a corn seedling stage and a use thereof. The method comprises disinfecting surfaces of corn seeds, and soaking the seeds in a Beauveria bassiana spore suspension, wherein the Beauveria bassiana strain is preserved in the China general microbiological culture collection center on January 23, 2013 and has an accession number of CGMCC7192. The method is simple and easy and ensures the optimal seed soaking time and colonization preference of Beauveria bassiana in a corn seedling stage. Through colonization of entomogenous fungi in a plant, plant resistance is improved so that pathogen-caused damage to plants is reduced and plant pests are avoided through indirect regulation and control of phytophagous insects.

Description

technical field [0001] The invention relates to the technical field of microbial colonization, in particular to a method for colonizing Beauveria bassiana at the seedling stage of corn and its application. Background technique [0002] Fungi that parasitize on insects, spiders and other larvae or adults are called entomogenous fungi (entomogenous fungi or entomopathogenic fungi). Entomogenous fungi play an important role in protecting plants from pests and diseases. Microbial taxa rapidly gaining attention. The plant endophytic nature of entomogenic fungi has been verified by many researchers. This type of fungi can exist in the tissues of many wild plants, such as corn, sorghum, tomato, cotton, banana, California mountain pine, cocoa, poppy, etc. In addition, the bacteria can also be inoculated into the host plant by artificial inoculation. The main inoculation methods include soil irrigation, seed coating, seed soaking, radicle dressing (soil seed dressing), root and rhiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08A01G7/06
CPCA01C1/08A01G7/06
Inventor 隋丽徐文静李启云王永志路扬张正坤
Owner JILIN ACAD OF AGRI SCI
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