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A preparation method of a compound that reacts with alkaline phosphatase to generate chemiluminescence

A phosphatase reaction and chemiluminescence technology, which is applied to phosphorus organic compounds, chemical instruments and methods, and luminescent materials, can solve the problems of poor stability of synthetic substances and complex synthesis processes, and achieve good stability, simple preparation process, and low price. cheap effect

Active Publication Date: 2019-01-04
湖南远璟生物技术有限公司
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  • Claims
  • Application Information

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Problems solved by technology

However, the synthesis process is relatively complicated, and the stability of the synthetic substance is relatively poor.

Method used

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  • A preparation method of a compound that reacts with alkaline phosphatase to generate chemiluminescence
  • A preparation method of a compound that reacts with alkaline phosphatase to generate chemiluminescence
  • A preparation method of a compound that reacts with alkaline phosphatase to generate chemiluminescence

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Embodiment Construction

[0024] Further illustrate the present invention below in conjunction with example, advantage and characteristic of the present invention will be described more clearly. However, it should be understood that this example is only an example of the present invention and does not limit the scope of the present invention in any way.

[0025] (1) Prepare the main experimental materials:

[0026] a. Acridine-9-carboxylic acid (≥97%, Sigma)

[0027] b. 4-Dimethylaminopyridine (≥99%, Sigma)

[0028] c. Thionyl chloride (AR, Sinopharm Group)

[0029] d. Dimethyl carbonate (≥99%, Sigma)

[0030] e. Cesium carbonate (AR, Sinopharm Group)

[0031] f. 3-ethoxythiophenol (≥97%, Sigma)

[0032] g. 3-Hydroxypropionitrile (≥99%, Sigma)

[0033] (2) Synthesis of acridine-9-acid chloride: Dissolve 2.53g of acridine-9-carboxylic acid in 10mL of chloroform in a 150mL three-neck reaction flask, add 1.25g of 4-dimethylaminopyridine, and slowly add sulfite Acyl chloride 5 mL and the reaction mi...

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Abstract

The invention relates to a preparation method of a compound reacting with alkaline phosphatase to create chemiluminiscence. The preparation method comprises the following synthesizing steps in sequence: (1) synthesizing acridine-9-sulfuryl chloride; (2) synthesizing 9, 10-acridanyl-9-sulfuryl chloride; (3) synthesizing 10-methyl-9, 10-acridanyl-9-sulfuryl chloride; (4) synthesizing 10-methyl-9, 10-acridanyl-9-thiocarboxylic acid-3'-ethylpatate; (5) synthesizing 9-(3'-phenetyl sulfurized phosphoricoxymethylene)-10-methyl-9, 10-acridanyl bi(cyanoethyl) ester. The preparation method is simple in technology, high in stability, and low in price.

Description

technical field [0001] The invention relates to a preparation process of a compound that reacts with alkaline phosphatase to generate chemiluminescence, and belongs to the technical field of chemiluminescence immunoassay. technical background [0002] Chemiluminescent enzyme immunoassay includes two parts: immunochemical reaction and chemiluminescent reaction. The immunoassay system is to label the enzyme on the antigen or antibody, and form the antigen-antibody immune complex through the specific reaction between the antigen and the antibody. The chemiluminescent analysis system is to add the luminescent substrate of the enzyme after the immune reaction is over. After the chemiluminescent substance is catalyzed by the enzyme, it will form an intermediate in an excited state, which will emit photons and release energy to return to a stable ground state. The luminous intensity can be Detection is performed using a luminescent signal measuring instrument. The detection purpo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/64C09K11/06
CPCC07F9/64C09K11/06C09K2211/1029
Inventor 李明勇姜雪莲龚小贵黄金浪曹文军黄伟
Owner 湖南远璟生物技术有限公司