Recombinant strain capable of producing tyrosol and construction method thereof

Abstract

The invention discloses a recombinant strain capable of producing tyrosol and a construction method thereof, and belongs to the field of microorganisms. The method comprises the following steps: constructing a recombinant carrier pRSFDuet-ARO10-ARO8 by a gene engineering technology; transforming the recombinant carrier into escherichia coli BL21(DE3) from which a gene pheA and a gene feaB are removed by gene engineering to obtain a strain BE0; carrying out a whole-cell catalysis reaction; fermenting for 20 hours by taking 10mN of tyrosine as a substrate to produce 6.76mM of tyrosol, wherein the tyrosine transformation ratio can be up to 67.3 percent; performing chemical mutagenesis on the basis of the recombinant strain to obtain a recombinant strain BE2 capable of producing tyrosol at high yield with optimized performance; carrying out a whole-cell catalysis reaction; fermenting for 20 hours by taking 10mN of tyrosine as a substrate to produce 8.71mM of tyrosol, wherein the tyrosine transformation ratio can be up to 87.1 percent.

Description

technical field [0001] The invention relates to a recombinant bacterial strain producing tyrosol and a construction method thereof, belonging to the field of microorganisms. Background technique [0002] Tyrosol (4-(2-Hydroxyethyl)phenol) is a natural antioxidant derived from olive oil and a derivative of phenylethyl alcohol. As an antioxidant, it protects cells from oxidative damage. For phenolic compounds, it has great industrial value. Tyrosol, and its derivatives, are synthons for the synthesis of various organic compounds. For example, tyrosol can be used to prepare commercially available pharmaceutical agents, such as, betaxolol and metoprolol, these agents are selective β1 receptor blockers, and can be used to treat hypertension, Angina, heart failure, and glaucoma. In addition, tyrosol may be the precursor of hydroxytyrosol. Hydroxytyrosol (2-(3,4-dihydroxyphenyl)ethanol) is an antioxidant beneficial to human health. Compared with tyrosol, its antioxidant Strong...

AI Technical Summary

Problems solved by technology

This process has high yield, but its disadvantage is that the price of raw material phenylethanol is expensive and the supply is tight

Other chemical synthesis processes, such as p-nitrotoluene synthesis method, have relatively low raw material prices, more sources of goods, and lower costs, but the steps are longer and the yield is low, which is not conducive to environmental protection; the yield of p-hydroxystyrene synthesis method is 96%. , with a purity of 99%, high yield and purity, and has certain value, but the large input of raw material costs also restricts large-scale industrial production

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