Capillary electrophoresis electrochemiluminescence detection method of flavonoids compound
A technology of flavonoids and capillary electrophoresis, which is applied in the fields of chemiluminescence/bioluminescence, analysis by causing chemical reactions of materials, and measurement devices, etc. It can solve the problems of large sample consumption and inability to analyze and detect precious trace samples.
Inactive Publication Date: 2017-05-31
SHAANXI SCI TECH UNIV
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Problems solved by technology
[0004] As far as the prior art is concerned, the analysis and detection of flavonoids usually use techniques such as ultraviolet spectrophotometry, high performance liquid chromatography, and liquid chromatography-mass spectrometry. However, these methods generally have the problem of large sample consumption, and cannot be used for some Analysis and detection of special and precious trace samples
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[0079] Capillary electrophoresis electrochemiluminescence detection method for cornflower-3-O-glucoside:
[0080] Currently, the main natural flavonoids are classified into: flavones, flavonols, flavonones, flavanonols, anthocyanidins, flavans -3, 4 diols (flavan-3,4-diols), xanthones (xanthones), chalcones (chalcones) and biflavonoids (biflavonoids) and other fifteen types; among them, anthocyanins in nature There are about 600 kinds of compounds;
[0081] Here we take the capillary electrophoresis electrochemiluminescence detection method of Cornflower-3-O-glucoside as an example for illustration, and the details are as follows:
[0082] Take 250μL of bluebonnet-3-O-glucoside standard mother solution of 100mg / L~200mg / L, and add 2500μL acetate buffer (0.4mol / L, pH 4.5) to it, and obtain solution I after mixing -A, use an ultraviolet spectrophotometer to scan the spectrum, such as figure 1 As shown in the control group Figure Ⅰ -A;
[0083] Take five parts of Cornflower-3-O-glucosi...
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The invention discloses a capillary electrophoresis electrochemiluminescence detection method of a flavonoids compound. The method comprises the following steps: adding acetate buffer liquid into a flavonoids compound standard substance and scanning the spectrum of the standard substance by a spectrophotometer to obtain a control group picture; performing reaction on five amine salts and the flavonoids compound standard substance mother liquor in the acetate buffer liquid separately, scanning a reactant by a spectrophotometer, and comparing a result with the control group picture; adding dipyridyl ruthenium electrochemical luminescence liquid into a capillary electrophoresis electrochemiluminescence detection pond, and detecting an electrochemical luminescence reference map by means of cyclic voltammetry; adding the dipyridyl electrochemical luminescence liquid into the capillary electrophoresis electrochemiluminescence detection pond and adding five solutions, detecting five control groups by means of cyclic voltammetry, and performing comparison to obtain a tertiary amine derived reaction reagent with the strongest electrochemical luminescence signal; and performing follow-up treatment to finish detection. The capillary electrophoresis electrochemiluminescence detection method disclosed by the invention has the advantages that the sample consumption is low and the analyzing result is not interfered by sample oxidization.
Description
Technical field [0001] The invention belongs to the technical field of compound detection methods, and specifically relates to a capillary electrophoresis electrochemiluminescence detection method for flavonoids. Background technique [0002] Flavonoids (flavonoids) are compounds that exist in nature and have a 2-phenylchromone (flavone) structure. They have a ketone carbonyl group in their molecule. The oxygen atom in the first position is basic and can interact with Strong acid forms salt, and its hydroxyl derivatives are mostly yellow, so it is also called flavone or flavonoid. Flavonoids are usually combined with sugars in plants to form glycosides, and a small part exists in the form of free (glycon). Most plants contain flavonoids, which play an important role in plant growth, development, flowering, fruiting, antibacterial and disease prevention. There are many compounds with medicinal value in flavonoids, such as: Xiangtianguo, rutin in Sophora japonicus and Chenpi glyc...
Claims
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Login to View More IPC IPC(8): G01N21/76G01N27/447
CPCG01N21/76G01N27/447
Inventor 陈志远
Owner SHAANXI SCI TECH UNIV