Paper-base micro-fluidic chip for hemoglobin detection in whole blood, and manufacture and application of paper-base micro-fluidic chip

Abstract

The invention discloses a paper-base micro-fluidic chip for hemoglobin detection in whole blood, and manufacture and application of the paper-base micro-fluidic chip. Various functional units, such as cytoplasm pre-separation, hemocyte elution, cell lysis and hemoglobin release, interference impurity removal and a three-electrode system, are processed on a paper base through silk-screen printing and laser engraving technologies; the chip comprises fan elution tanks with laser etching array microfractures with different densities, which are used for multiple speedy washing of hemocyte and hemoglobin by different eluents; the chip accelerates the electron transfer between the hemoglobin and electrodes through a polishing carbon working electrode simply and physically modified by methylene blue, so that the three-electrode system has large current response and detection ranges; the three-dimensional paper-base micro-fluidic chip with a three-dimensional structure through simple superposition and partial turnover of the functional units in different zones of the chip can be obtained; the while detection process is simple in operation, the analysis speed is high, the size of a sample required is small, and the chip is applicable to rapid detection of hemoglobin content in whole blood for family practice, field emergency treatment in remote areas and underdeveloped areas.

Description

technical field [0001] The invention relates to the technical field of microfluidic analysis applied to the quantitative determination of hemoglobin in whole blood, in particular to a paper-based microfluidic chip for detecting hemoglobin in whole blood and its production and application. Pre-separation, blood cell elution, cell lysis and release of hemoglobin, removal of interfering impurities (inorganic salts, glucose and lipids) and other sample pretreatment units, integrated with the screen printing three-electrode system for cyclic voltammetry electrochemical detection Paper-based microfluidic chip for blood hemoglobin detection. Background technique [0002] As a component of blood, hemoglobin is responsible for carrying oxygen from the lungs to various organs and tissues of the body, and has very important physiological functions (A.N. Schechter, Blood, 2008, 112, 3927). Too high or too low hemoglobin content will lead to the reduction of blood oxygen transport capac...

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Problems solved by technology

However, the above research uses glassy carbon electrodes, silver electrodes, and gold electrodes with high cost, and the electrode modification process is relatively complicated. The detection results of some methods depend on the previous sample pretreatment effect, and the traditional independent electrode system is not conducive to the three-electrode system. Integration and mass production

[0003] Due to the advantages of simple preparation process, easy operation, low cost, portability, environmental friendliness, micro-integration, and fast analysis speed, paper-based electrochemical analysis chips have developed rapidly in recent years, and are widely used in small life in homes, fields, and areas where resources are scarce. Rapid detection of molecules, cancer markers, pollutants, pathogens, etc., but its detection of hemoglobin in whole blood has not been reported

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