Method for detecting polycyclic aromatic hydrocarbon in tea oil

Abstract

The invention discloses a method for detecting polycyclic aromatic hydrocarbon in tea oil. The method comprises the following steps: adding a magnetically hydroxylated multiwall carbon nano tube serving as a solid phase extraction reagent into the tea oil diluted by a normal hexane solvent for adsorption of a target substance, carrying out magnetic separation under the action of an external magnetic field, collecting the solid phase extraction reagent with the target substance, removing supernate, purifying the solid phase extraction reagent with the target substance through a purifier, desorbing the target substance on the solid phase extraction reagent through methylbenzene serving as a desorbent, carrying out magnetic separation under the action of the external magnetic field, removing the solid phase extraction reagent, collecting a desorption solution with the target substance, filtering the desorption solution through an organic filter film, and carrying out conventional content measurement by combining gas chromatography and mass spectrum. The method is high in selectivity, low in detection limit, high in sensitivity and high in standard recovery rate.

Description

technical field [0001] The invention relates to a method for detecting polycyclic aromatic hydrocarbons in edible oil, in particular to a method for detecting polycyclic aromatic hydrocarbons in camellia oil. Background technique [0002] Polycyclic aromatic hydrocarbons are lipophilic compounds, and edible oil is the main source of food transmission. Once polycyclic aromatic hydrocarbons enter the human body through the food chain and participate in the metabolism of the body, they are likely to cause toxic and side effects on human fertility, development, internal organs, nervous and immune systems, etc. In a high PAHs environment, it is also easy to induce lung cancer, skin cancer and other cancers. More than 400 carcinogenic PAHs and their derivatives have been discovered so far, and they are the earliest and most numerous carcinogens. [0003] It is well known that high fat content can make the analysis and detection of low concentration residues in edible oil matrix d...

AI Technical Summary

Problems solved by technology

Due to their high hydrophobicity, PAHs exhibit a strong affinity for oils, so the extraction of PAHs from edible oils usually requires a lot of manpower and time.

At present, there are few pretreatment methods for the detection of PAHs in camellia oil with high nutritional value unique to my country, but the main pretreatment methods for PAHs in olive oil, soybean oil and other edible vegetable oils include liquid-liquid extraction, solid-phase extraction, and solid-phase extraction. Extraction, solid-phase microextraction, gel permeation chromatography, the process of concentrating the extract in the first two methods is more time-consuming; while solid-phase microextraction requires a longer adsorption equilibrium process, and gel permeation chromatography requires more organic solvents, etc., but these traditional pretreatment methods cannot meet the characteristics of simple, fast, economical and efficient detection of PAHs in camellia oil