Luciferase co-immunoprecipitation kit for detecting mammalian lyme diseases
A co-immunoprecipitation and luciferase technology, applied in the field of luciferase co-immunoprecipitation kits, can solve the problems of complex operation, large impact, and low repeatability of experimental results, and achieve high sensitivity, high signal-to-noise ratio, Stable and reliable results for measured values
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[0041] A luciferase immunoprecipitation kit for detecting Lyme disease in mammals, comprising the following components: (1) sample dilution; (2) Renilla luciferase and Borrelia burgdorferi surface membrane protein OspC antigen The composition of the fusion protein solution; (3) proteinA / G coated ELISA plate; (4) washing solution; (5) luciferase substrate; (6) substrate dilution. Among them, (5) luciferase substrate and (6) substrate dilution are all from Renilla Luciferase Assay Systerm (Renilla Luciferase Assay Systerm, Promega Biotechnology Co., Ltd.), catalog number E2810.
[0042] The composition of the sample diluent is as follows: 20mM Tris pH7.5, 150mM NaCl, 5mM MgCl 2 , 1% TritonX-100, water balance.
[0043] The washing liquid preparation method is: NaCl 8g, KCl 0.2g, Na 2 HPO 4 ·12H 2 O 3.63g, KH 2 PO 4 0.24g, dilute to 1L with water, and sterilize.
[0044] The fusion protein solution is prepared by the following method:
[0045] a. The OspC antigen gene ...
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