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Method for extracting xylan from wheat brans

A technology for wheat bran and xylan, which is applied to the field of xylan extraction from wheat bran, can solve the problems of residual xylan impurities, easily destroy xylan molecules, cannot meet needs, etc., and achieves good stability, High purity, suitable for accurate measurement

Active Publication Date: 2017-06-06
北京挑战生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nowadays, there are relatively mature techniques for extracting xylan from wheat bran, including high-temperature alkali method or ultrasonic-assisted extraction, etc., but high-temperature and ultrasonic methods are easy to destroy xylan molecules, and the produced xylan impurities remain a lot , cannot meet the needs

Method used

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  • Method for extracting xylan from wheat brans
  • Method for extracting xylan from wheat brans
  • Method for extracting xylan from wheat brans

Examples

Experimental program
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Effect test

Embodiment 1

[0065] Example 1: Extraction and Extraction Rate Determination of Wheat Bran Xylan from Beijing, the Place of Origin

[0066]Wheat bran pretreatment: take 200-300g of wheat bran originating in Beijing, grind them, and pass through a 60-mesh sieve. Wrap the sieved wheat bran with fine-meshed cotton cloth, add trypsin, tie it tightly with a rubber band, wash it in hot water several times and wring it dry. Put the destarched bran into a 5L beaker, add 0.2% (w / v) EDTA solution, stir for 1h, filter with fine-pored cotton cloth (4.1), wash with distilled water and wring dry. Dry at 105°C for 4 hours, crush and save.

[0067] Extraction of xylan: Weigh an appropriate amount of pretreated wheat bran in alkali solution, add hydrogen peroxide solution, the ratio of solid to liquid is 1:50, add 4M NaOH solution to adjust the pH to alkaline while stirring magnetically, heat and shake in a constant temperature water bath overnight . Then use 10% hydrochloric acid to adjust the pH value ...

Embodiment 2

[0068] Embodiment 2: the wheat bran xylan extraction of place of origin Jiangsu

[0069] Except that the wheat bran was sourced from Jiangsu, other parameters were the same as in Example 1, and the extraction rate was determined to be 31.07% according to the above formula.

Embodiment 3

[0070] Example 3: Extraction of wheat bran xylan from Jilin, the place of origin

[0071] Except that the wheat bran was from Jilin, other parameters were the same as in Example 1, and the extraction rate was determined to be 32.09% according to the above formula.

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Abstract

The invention discloses a method for extracting xylan from wheat brans. The method comprises the following steps: crushing and sieving of wheat brans, de-starching pretreatment, alkali dissolution with sodium hydroxide, adding of hydrogen peroxide to remove lignin, neutralization, filtration, precipitation with organic solvent, and drying to prepare a xylan substrate product. The method provided by the invention is simple in process, mild in condition and good in extraction purity, has the extraction rate up to 30%, has extraction rate variation coefficients in the same batch and different batches not greater than 8%, has enzyme activity variation coefficients for measurement of xylanase substrate in feed not greater than 8%, has good repeatability, and has high application and promotion values.

Description

technical field [0001] The invention belongs to the technical field of plant xylan production, in particular to a method for extracting xylan from wheat bran. Background technique [0002] Xylan (xylan) is a heterogeneous polysaccharide existing in plant cell walls, accounting for about 15% to 35% of the dry weight of plant cells, and is the main component of plant hemicellulose. Most xylans are complex, highly branched heterogeneous polysaccharides with many different substituents, which are widely used. [0003] Recently, xylan is regarded as one of the most important renewable resources because it plays an important role in the new biopolymer materials and functional polymers obtained through chemical modification reactions. Xylan is also favored because of the unlimited application potential of the diversity of functional molecules. The most likely important applications of xylan and its derivatives are drug carriers, wound dressings, and additives in the paper industry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 严峰杨昕冉王海燕张广民
Owner 北京挑战生物技术有限公司
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