In situ molding injectable bioactive composite hydrogel and its preparation method and application

A composite hydrogel, in-situ molding technology, applied in the field of biomedical materials, can solve the problems of poor injectability and lack of biological activity of sodium alginate, and achieve high water content, good biocompatibility, and improved mechanical strength. Effect

Active Publication Date: 2019-08-16
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate (SA) hydrogel is one of the more mature hydrogel systems currently studied, and it has been applied in tissue engineering, but traditionally, sodium alginate solution is added dropwise to the multivalent cation solution, Because the gel is formed too quickly from the outside to the inside, the prepared sodium alginate hydrogel beads have poor injectability and the sodium alginate itself lacks biological activity

Method used

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  • In situ molding injectable bioactive composite hydrogel and its preparation method and application
  • In situ molding injectable bioactive composite hydrogel and its preparation method and application
  • In situ molding injectable bioactive composite hydrogel and its preparation method and application

Examples

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Effect test

preparation example Construction

[0059] In one example, a method for preparing a composite hydrogel includes the following steps:

[0060] a. Stir and dissolve sodium alginate powder at room temperature, with a mass volume concentration of 1.5%-2%, and overnight at 4°C to make it dissolve evenly;

[0061] b. Weigh a certain amount of silicate bioceramic powder, add it into the sodium alginate solution to make its mass volume concentration 1%-20%, and stir evenly;

[0062]c. Put the mixed solution formed in b into the syringe on one side of the three-way pipe, and add a certain amount of amino acid powder into the syringe on the other side according to the required gel time, and make the mass volume concentration of the amino acid At 0.25%-2%, the two are mixed evenly in the tee pipe;

[0063] d. During the mixing process, the hydrolysis of amino acids will promote the slow release of multivalent cations in the silicate bioceramic powder inside the sodium alginate, and the sodium alginate will be cross-linked...

Embodiment 1

[0068] Example 1 Injectable bioactive sodium alginate / magnesia feldspar bioceramic / glutamic acid composite hydrogel

[0069] a. Stir and dissolve sodium alginate powder (SA, sigma, product number A2033) at room temperature, with a mass volume concentration of 1.5%, and overnight at 4°C to make it dissolve evenly;

[0070] b. Weigh a certain amount of feldspar bioceramic powder (Aker, synthesized and prepared by our research group), add it to the sodium alginate solution, make its mass volume concentration 2%, and stir evenly;

[0071] c. Put the mixed solution formed in b into the syringe on one side of the tee tube, and add a certain amount of glutamic acid powder (Glu, sigma, Cat. No. 49449), and the mass volume concentration of glutamic acid is 0.5%, and the two are mixed uniformly in the three-way pipe;

[0072] d. During the mixing process, the hydrolysis of glutamic acid will promote the magnesia feldspar bioceramic powder to slowly release Ca and Mg ions inside the sod...

Embodiment 2

[0073] Example 2 Injectable bioactive sodium alginate / magnesia feldspar bioceramic / glutamic acid composite hydrogel

[0074] a. Stir and dissolve sodium alginate powder at room temperature, with a mass volume concentration of 1.5%, overnight at 4°C to make it dissolve evenly;

[0075] b. Weigh a certain amount of feldspar bioceramic powder, add it into the sodium alginate solution, make its mass volume concentration 20%, and stir evenly;

[0076] c. Put the mixed solution formed in b into the syringe on one side of the tee pipe, and add a certain amount of glutamic acid powder into the syringe on the other side according to the required gel time, and make the glutamic acid The mass volume concentration is 0.25%, and it is mixed uniformly in the three-way pipe;

[0077] d. During the mixing process, the hydrolysis of glutamic acid will promote the magnesia feldspar bioceramic powder to slowly release Ca and Mg ions inside the sodium alginate, and the sodium alginate will be cr...

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Abstract

The invention relates to in-situ forming injectable bioactive composite hydrogel as well as a preparation method and application thereof. The composite hydrogel is formed by mixing sodium alga acid, amino acids and silicate bioceramics containing two or more than two multivalent cations serving as raw materials and performing in-situ forming, wherein the raw materials respectively comprise the following components by weight volume: 1.5-2% of sodium alga acid, 1-20% of silicate bioceramics and 0.25-2% of amino acids. According to the composite hydrogel disclosed by the invention, the inorganic component silicate bioceramics have the effect of promoting cell migration, bone formation, vascularization and other biological activities and can serve as a reinforced phase inside the hydrogel for improving the mechanical strength of the composite hydrogel, so that the composite hydrogel can provide a physical support and a good chemical environment for regeneration and reconstruction of tissues.

Description

technical field [0001] The invention belongs to the fields of biomedical material technology, tissue defect filling, wound repair and medical equipment, and specifically relates to an in-situ molded injectable bioactive composite hydrogel material containing an inorganic / organic phase, and the invention also relates to the composite hydrogel Preparation method and application of gel material. Background technique [0002] Defects of human tissues and organs appear in a variety of clinical situations, which seriously threaten the health of patients and reduce the quality of life of patients. It can be caused by various diseases such as tumors, trauma, severe infections or congenital defects. Although the assistance of medical equipment can effectively alleviate the suffering of patients, it cannot fundamentally repair the damage. Tissue damage can be reconstructed by transplanting corresponding tissue parts. These early transplants include autologous and allogeneic / xenograft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/52A61L27/50A61L27/56A61L27/54A61L15/28A61L15/20A61L15/18A61L15/42A61L15/44A61L26/00
CPCA61L15/18A61L15/20A61L15/28A61L15/425A61L15/44A61L26/0066A61L26/008A61L26/0085A61L26/0095A61L27/446A61L27/50A61L27/52A61L27/54A61L27/56A61L2300/102A61L2300/404A61L2300/412A61L2400/06A61L2430/00C08L5/04
Inventor 常江韩彦李勇辉
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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