Diluent for live vaccine for hog cholera

A technology of live swine fever vaccine and diluent, which is applied in the field of diluent for live swine fever vaccine, can solve the problems of reducing the immune effect of live swine fever vaccine, the uniformity of immune titers is not high, and the difference between vaccines is large, so as to improve the overall Immunization levels, reduced batch-to-batch variability, low-cost effects

Inactive Publication Date: 2017-06-13
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Live swine fever vaccine needs to be diluted with vaccine diluent when used. At present, water for injection or normal saline is usually used. However, the immune titer of the vaccine diluted in this way is not high. There are stress reactions and allergic reactions after inoculation of diseased pigs, which greatly reduces the immune effect of live swine fever vaccine

Method used

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  • Diluent for live vaccine for hog cholera
  • Diluent for live vaccine for hog cholera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Prepare Agaricus blazei polysaccharide, comprising the following steps:

[0025] (1) Grinding Agaricus blazei to obtain Agaricus blazei powder, according to the ratio of material to liquid: 1:70g / ml, add distilled water to Agaricus blazei powder and adjust the pH to 7.0;

[0026] (2) Microwave extraction: set the microwave power to 500W, extract for 8 minutes, and collect the extract. The microwave extraction process is intermittent microwave extraction, that is, after 2 minutes of extraction, pause for 1 minute, and at the same time add the lost water to keep the volume of the extract relatively constant. ;

[0027] (3) Repeat step (2) to collect the extract;

[0028] (4) Mix the filtrate obtained in steps (2) and (3), concentrate to 1 / 4 volume with a rotary evaporator, add 80% ethanol, let stand at 4 °C for 12 h, and collect the precipitate by centrifugation;

[0029] (5) The precipitate obtained in step (4) is washed with absolute ethanol and acetone in sequence...

Embodiment 2

[0039] 1. Prepare Agaricus blazei polysaccharide, comprising the following steps:

[0040] (1) Grind Agaricus blazei to obtain Agaricus blazei powder, according to the ratio of material to liquid: 1:80g / ml, add distilled water to Agaricus blazei powder to adjust the pH to 7.0;

[0041] (2) Microwave extraction: set the microwave power to 700W, extract for 10 minutes, and collect the extract. The microwave extraction process is intermittent microwave extraction, that is, after 2 minutes of extraction, pause for 1 minute, and at the same time add the lost water to keep the volume of the extract relatively constant. ;

[0042] (3) Repeat step (2) to collect the extract;

[0043] (4) Mix the filtrate obtained in steps (2) and (3), concentrate to 1 / 4 volume with a rotary evaporator, add 80% ethanol, let stand at 4 °C for 12 h, and collect the precipitate by centrifugation;

[0044] (5) The precipitate obtained in step (4) is washed with absolute ethanol and acetone in sequence, a...

Embodiment 3

[0054] 1. Prepare Agaricus blazei polysaccharide, comprising the following steps:

[0055] (1) Grind Agaricus blazei to obtain Agaricus blazei powder, add distilled water to Agaricus blazei powder according to the material-to-liquid ratio of 1:90g / ml and adjust the pH to 7.0;

[0056](2) Microwave extraction: set the microwave power to 600W, extract for 12 minutes, and collect the extract. The microwave extraction process is intermittent microwave extraction, that is, after 2 minutes of extraction, pause for 1 minute, and at the same time add the lost water to keep the volume of the extract relatively constant. ;

[0057] (3) Repeat step (2) to collect the extract;

[0058] (4) Mix the filtrate obtained in steps (2) and (3), concentrate to 1 / 4 volume with a rotary evaporator, add 80% ethanol, let stand at 4 °C for 12 h, and collect the precipitate by centrifugation;

[0059] (5) The precipitate obtained in step (4) is washed with absolute ethanol and acetone in sequence, and...

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Abstract

The invention belongs to the technical field of biological products for animals, and particularly relates to diluent for a live vaccine for hog cholera. 5g to 10g of agaricus blazei polysaccharide, 1g to 5g of herba andrographitis, 0.1g to 2g of ginsenoside Rh2 and 2g to 6g of curcumin are contained in every 1,000ml of a phosphate buffer solution, the pH (potential of Hydrogen) of which is equal to 7, of the diluent. The diluent for the live vaccine for the hog cholera, which is provided by the invention, is not only convenient to produce, low in cost and convenient and safe to use, but also can be used for improving the conversion ratio of a lymphocyte, decreasing a difference among vaccines of different batches and improving the immunization overall effect of the vaccines.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a diluent for swine fever live vaccine. Background technique [0002] Classical swine fever virus (CSFV) is a highly contagious infectious disease caused by classical swine fever virus (CSFV). It is characterized by bleeding and fever and can be acute or chronic. It is a very harmful infection to pigs. sick. Pigs of different ages, sexes and breeds can be infected with the disease, and the mortality rate is as high as 80-90%. Swine fever is classified as a class A disease by the World Organization for Animal Health, and it is classified as a class I disease in my country. At present, vaccination is an important means of controlling swine fever in many countries and regions in the world. [0003] Live swine fever vaccine needs to be diluted with vaccine diluent when used. At present, water for injection or normal saline is usually used. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K47/36A61K47/46A61K47/28A61K47/10A61K36/19A61P37/04A61K31/715A61K31/704A61K31/12
CPCA61K9/08A61K31/12A61K31/704A61K31/715A61K36/19A61K47/10A61K47/28A61K47/36A61K47/46A61K2300/00
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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