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Perfluoro polyether type fluorocarbon surfactant as well as preparation method and application thereof

A perfluoropolyether, fluorocarbon surface technology, applied in biochemical equipment and methods, DNA preparation, chemical instruments and methods, etc., can solve the problems of weak hydroxyl reaction activity and low reaction yield, and achieve low cost and purification. Simple, easy-to-synthesize effects

Active Publication Date: 2017-06-13
广东永诺医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the synthetic route in this method is simplified, the reactivity of the hydroxyl group is weaker than that of the amino group, resulting in a very low reaction yield

Method used

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  • Perfluoro polyether type fluorocarbon surfactant as well as preparation method and application thereof
  • Perfluoro polyether type fluorocarbon surfactant as well as preparation method and application thereof
  • Perfluoro polyether type fluorocarbon surfactant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A perfluoropolyether fluorocarbon surfactant is synthesized and prepared according to the following route:

[0044]

[0045] (1) Acylation.

[0046] First weigh 0.729g of carboxyl perfluoropolyether (PFPE-COOH) (purchased from DuPont, Krytox157FSH, average molecular weight is 6500) dissolved in 50ml of solvent fluorine oil (purchased from 3M company, model is Novec TM 7100), then add thionyl chloride 0.16ml (about 20 equivalents), and stir and reflux overnight (about 12h) under the protection of nitrogen.

[0047] After the mixture was cooled to room temperature, it was concentrated by distillation with a rotary evaporator to obtain acylated perfluoropolyether (PFPE-COCl), and the solvent and excess thionyl chloride were removed by vacuuming.

[0048] (2) Reaction.

[0049] Dissolve PFPE-COCl in 80ml of (Novec TM 7100: trifluorotoluene=5: 3) in the reaction solvent, under nitrogen protection, add 0.729g chitosan (this chitosan is 1 times of PFPE-COOH quality, deac...

Embodiment 2

[0056] A droplet forming oil, the fluorosurfactant prepared in Example 1 was dissolved in 3M in an amount of 2% by mass TM Novec TM 7500 fluorine oil, that is.

Embodiment 3

[0058] Application of the droplet forming oil of Example 2 in the microdropletization process of PCR amplification.

[0059] PCR oil phase: the droplet forming oil in Example 2 was used as the PCR oil phase.

[0060] PCR water phase: The PCR water phase includes (40-60) 2X Buffer, (0.02-0.06) gDNA (20ng / μL), (0.006-0.01) Taq (5U / μL), (0.02-0.06) Primer-F ( 10 μM), (0.02-0.06) Primer-R (10 μM), (0.01-0.03) Probe (10 μM). The above solutions were uniformly mixed sequentially, and ultrapure water was added dropwise and mixed uniformly as the reaction aqueous phase for later use.

[0061] Take 7 times the volume of the oil phase and 2 times the volume of the water phase to generate well-uniform water-in-oil droplets (20 μm-100 μm) in the microfluidic chip, such as Figure 4 shown.

[0062] Then PCR amplification thermal cycling experiments were performed. The droplet morphology was observed under the microscope before thermal cycling of PCR amplification, such as Figure 5 As...

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Abstract

The invention relates to a perfluoro polyether type fluorocarbon surfactant as well as a preparation method and application thereof, and belongs to the technical field of high-molecular surfactants. The perfluoro polyether type fluorocarbon surfactant with structural characteristics shown in a formula I can be used as an oil phase component in a PCR method and is applied o droplet type digital PCR, and the droplet stability can be ensured; the perfluoro polyether type fluorocarbon surfactant has excellent surface performance.

Description

technical field [0001] The invention relates to the technical field of polymer surfactants, in particular to perfluoropolyether fluorocarbon surfactants and their preparation methods and applications. Background technique [0002] Fluorocarbon surfactants are the most important species of special surfactants, and are the class of surfactants with the highest surface activity so far. The surface tension of water or organic solvents can be reduced to a very low value when used in a small amount. Its unique properties are often summarized as "three highs, two ", that is, high surface activity, high thermal stability and high chemical stability, and its fluorine-containing hydrocarbon group is both water again Oil. [0003] Among perfluoropolyether surfactants, the general structural formula of perfluoropolyether is as follows: [0004] [0005] In general, n / m=20-40 in the formula, R f It is a C4-C3 fluorocarbon terminal chain. [0006] Currently commonly used perf...

Claims

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Application Information

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IPC IPC(8): B01F17/42C08G81/00C12N15/10C09K23/42
CPCC12Q1/686C08G81/00C09K23/16
Inventor 何蕾李家玉古晓奎
Owner 广东永诺医疗科技有限公司
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