Affinity peptides associated with cell membrane markers

A cell membrane and cell technology, applied in the field of bioengineering pharmaceuticals, can solve problems such as the integrity of cell functions, long fluorescence decay time, unfavorable normal metabolism, etc., and achieve the effects of low immunogenicity, low synthesis cost, and convenient access channels

Active Publication Date: 2020-06-30
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The longer fluorescence decay time can only be used for long-term labeling observation in in vivo detection, and the drug metabolism time is often completed in a short time. Long-term cell fluorescence labeling is not conducive to the normal metabolism of the body
Although the Di series dyes have the advantages of low fluorescence variability, good labeling stability, low cytotoxicity and little cell-to-cell transfer, when the drug is metabolized by the cell, the integrity of the labeled cell function is affected

Method used

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  • Affinity peptides associated with cell membrane markers
  • Affinity peptides associated with cell membrane markers
  • Affinity peptides associated with cell membrane markers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The synthetic process of polypeptide H4 of the present invention

[0052] 1: Coupling

[0053] First, swell 0.33 / 2mmol6.06g of Fmoc-Arg-Rink amide MBHA resin (fluorenyl methaneoxycarbonyl-arginine-rink amino resin) in dimethylformamide, 10ml / g resin, and then use 20% hexahydro Pyridine / dimethylformamide solution removes the Fmoc protecting group (hereinafter referred to as decapping), washes the resin after decapping with dimethylformamide, adds raw material Fmoc-Lys(Boc)-OH 2.3g, and is used for condensing agent HBTU 1.44g, reaction time 30 minutes, kaiser method detection, if the test result is negative, indicating that the coupling reaction is complete, then proceed to uncapping for 30 minutes, if the test result is still positive, it indicates that the coupling reaction is not complete or The reaction is not complete, it is necessary to prolong the coupling time or re-feed the coupling until the coupling reaction is completed. Repeat the above operations and perfo...

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PUM

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Abstract

The invention discloses a series of affinity polypeptides related with cell membrane labeling SEQ ID NO:1. The polypeptides is likely to achieve the near infrared imaging of specific malignant tumors for cell membrane binding property. The polypeptides can be used for labeling, diagnosis of tumors, or preparation of tracer drugs, targeted chemotherapy, and drug auxiliary material or connectors, and therefore the goal of the real-time, lossless, in-situ monitoring of drug going in and out of the cells can be achieved. The invention relates to the field of nano-medicine related with body dynamics monitoring, in particular to multiple polypeptides, affinity cell test thereof, and a medicine combination using the polypeptides as the effective ingredient, and the application thereof in the preparation of tracer drugs.

Description

technical field [0001] The invention belongs to the technical field of bioengineering pharmacy and the field of protein polypeptide medicine and biomedical engineering, and specifically relates to a polypeptide with high affinity for cell membranes and its application, for example, for in vivo detection of nanomedicine. Background technique [0002] The cell membrane or plasma membrane plays an important role in the "social behavior" of the cell population, such as intercellular communication, cell movement and migration, cell adhesion, material exchange with the extracellular environment, and recognition by the body's immune mechanism. All are related to the function of the plasma membrane. Many growth characteristics and invasion and metastasis abilities of malignant tumor cells are also related to the changes of plasma membrane. [0003] Compared with normal cells, an important characteristic of the chemical composition of tumor cell membranes is that the content of mono...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12Q1/02A61K48/00A61K38/10A61P35/00
CPCA61K38/00A61K48/0008C07K7/08G01N33/5005
Inventor 顾月清韩智豪芮雅兰
Owner CHINA PHARM UNIV
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