Mitochondria-target fluorescent carbon dot as well as preparation method and application thereof

A technology of fluorescent carbon dots and mitochondria, which is applied in chemical instruments and methods, preparations for in vivo experiments, luminescent materials, etc. Low toxicity, good water solubility, small size effect

Active Publication Date: 2017-06-13
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the ligand is highly toxic and does not have fluorescent properties, it is usually necessary to introduce other fluorescent molecules during the modification process to realize the fluorescent tracking of the drug...

Method used

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  • Mitochondria-target fluorescent carbon dot as well as preparation method and application thereof
  • Mitochondria-target fluorescent carbon dot as well as preparation method and application thereof
  • Mitochondria-target fluorescent carbon dot as well as preparation method and application thereof

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Embodiment 1

[0044] The preparation of fluorescent carbon dots in this embodiment comprises the following steps:

[0045] (1) Preparation of raw materials: Weigh chitosan, mercaptosuccinic acid and ethylenediamine so that the mass ratio is 5:2:1. The three are completely dissolved in dilute hydrochloric acid solution, then fully mixed and transferred to a hydrothermal In the reactor;

[0046] (2) Reaction: React in a hydrothermal reactor at 180°C for 24 hours to form a carbon dot solution;

[0047] (3) Purification: Centrifuge or filter to obtain the target fluorescent carbon dot solution.

[0048] For a schematic diagram of the reaction, see figure 1 , the transmission electron microscopy results of the prepared fluorescent carbon dots are shown in figure 2 , the UV–Vis absorption spectrum of the prepared fluorescent carbon dots is shown in image 3 , the fluorescence emission spectra of the prepared fluorescent carbon dots under excitation of different wavelengths are shown in Fig...

Embodiment 2

[0050] The preparation steps of embodiment 2 fluorescent carbon dots are the same as in embodiment 1, except that the mass ratio of chitosan, mercaptosuccinic acid and ethylenediamine in step (1) is 1:1:1.

Embodiment 3

[0051] Example 3 The preparation steps of fluorescent carbon dots are the same as in Example 1, except that the mass ratio of chitosan, mercaptosuccinic acid and ethylenediamine in step (1) is 1:1:10.

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Abstract

The invention discloses a mitochondria-target fluorescent carbon dot as well as a preparation method and application thereof. The fluorescent carbon dot is mainly prepared from the following raw materials in parts by weight: 1-20 parts of chitosan, 1-10 parts of ethylenediamine and 1-10 parts of mercaptobutanedioic acid; and the carbon dot with excellent fluorescence property is prepared from the chitosan, the ethylenediamine and the mercaptobutanedioic acid as raw materials through a hydrothermal method in one step. Compared with the prior art, the carbon dot is capable of achieving fast, no-clean and long-term photostable (anti-bleaching) imaging on mammalian cell mitochondria, and can be used for directionally conveying an anti-tumor drug to the mitochondria as a carrier, thereby achieving mitochondria-based targeted cancer therapy. In addition, the carbon dot also has the advantages of low preparation cost, good water dispersibility and low cytotoxicity, and hopefully replaces a commercial mitochondria fluorescent probe widely used at present.

Description

technical field [0001] The invention discloses a mitochondria-targeted fluorescent carbon point, a preparation method and an application thereof, and belongs to the technical field of bionano materials. Background technique [0002] Whether in mammalian normal cells or diseased cells, mitochondria have a profound impact on the life activities of cells with their unique functions, such as ATP production and cell energy supply, calcium ion signals inside cells and the generation of free radicals, The regulation of apoptosis and the maintenance of the homeostasis of the internal environment of cells, etc. Since mitochondria are of great significance to cell metabolism, the dysfunction of mitochondria is closely related to the occurrence and development of diseases such as diabetes, tumors, Parkinson's disease and Alzheimer's disease. Therefore, the observation and research of the number and shape of mitochondria are very important for detecting the state and life activities of...

Claims

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Application Information

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IPC IPC(8): C09K11/65A61K49/00A61K47/02
CPCA61K47/02A61K49/0019A61K49/0071A61K49/0097C09K11/65
Inventor 吴富根华先武鲍琰雯
Owner SOUTHEAST UNIV
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