Evaluation method of grape canker pathogenicity to grape

Abstract

The invention discloses a method for evaluating the pathogenicity of grape canker bacteria on grapes. The method is: take the bacterial strains cultured on the PDA plate, take the bacteria cake and place it in the improved Fries liquid medium for static culture for 14 days, centrifuge to collect the supernatant, filter with a pinhole filter to remove mycelia, spores and bacterial contamination , that is, the preparation of the crude toxin stock solution is completed. Select new young leaves of annual green branches, after surface disinfection, take out a leaf disc with a diameter of 10mm, absorb 10 μL of crude toxin stock solution and inoculate it in the central part of the leaf disc, measure the lesion area after 3 days, and evaluate the potency of grape canker sore crude toxin stock solution pathogenicity. The invention has the advantages of simple operation, low cost and good repeatability, and provides technical support for the research on the effect of the mycotoxin in the pathogenic process of grape canker bacterium and the research on the selection of grape varieties resistant to grape canker.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, and relates to an evaluation method of grape canker pathogenicity to grapes, in particular to four kinds of grape canker bacteria (Botryosphaeria dothidea, Lasiodiplodiatheobromae, Thielavia minor neofusicoccum parvum, Diplodia seriata) rough Preparation of toxin stock solution and evaluation method of pathogenicity. Background technique [0002] Grape canker caused by Botryosphaeria spp. fungi is an important grape disease. Grape canker mainly occurs on branches, leaves and fruits, and is manifested as tree vigor attenuation, canker spots on branches, shrunken leaves, and fruit shedding. So far, 21 species of Botryobacteriaceae have been found to cause grape canker, and they occur widely in more than 20 countries such as the United States, Japan, France, and New Zealand (URbez-Torres J R, Peduto F, Striegler RK, et al.Characterization of fungal pathogens associated with grapevine trun...

AI Technical Summary

Problems solved by technology

[0004] At present, the main methods for evaluating the pathogenicity of grape canker bacterium require the inoculation of live bacteria, and the implementation requires a large amount of plant materials and pathogenic bacteria, which is easy to contaminate and takes a long time.

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