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Enzyme-responsive intelligent bacterial adhesion resistant and bactericidal layer-by-layer self-assembled multi-layer film coating and preparation method thereof

A layer-by-layer self-assembly, anti-bacterial adhesion technology, applied in coatings, pharmaceutical formulations, medical science, etc., can solve the problems of no anti-bacterial adhesion, poor biocompatibility, no sterilization, etc., to improve anti-bacteria The ability of bacteria to adhere, reduce frictional resistance, and improve the effect of bactericidal ability

Inactive Publication Date: 2017-06-16
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the antibacterial surface and interface properties, antibacterial materials can be divided into antibacterial adhesion surface, contact sterilization surface and antibacterial release surface. Such coatings do not have a bactericidal function; contact bactericidal surfaces include grafted quaternary ammonium salts, quaternary phosphates, pyridinium salts, etc. The advantages of this type of antibacterial surface are firm coating and strong antibacterial ability, but the problem is that the cells High toxicity and poor biocompatibility
This type of coating has a certain slow-release and antibacterial ability, but does not have the ability to resist bacterial adhesion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Add 100ml of deionized water or super ionized water (pH=7.0) to a 250ml small beaker, magnetically stir, slowly add 0.1g of hyaluronic acid, the concentration is 1mg / ml, while stirring, slowly add hydrochloric acid solution to adjust the pH to 2.9 and continue Stir until it dissolves, and also adjust the chitosan solution with pH=2.9 and polylysine with pH=9.5, which is the coating liquid, and at the same time adjust the corresponding washing liquid with the same pH. The silicone gel sheet is cut into 1.5×2.5cm by a blade 2 Size, used as a substrate, ultrasonic cleaning with water, N 2 Blow dry. Clamp the silicone gel sheet with tweezers and immerse it in 5mg / ml PEI solution for 2h for pretreatment. Take out the pretreated silicone gel and soak it back and forth in HA and CHI 10 times for 8 minutes each time. After taking it out, soak it in lotion for 1 ~2s, blow dry with N2, then soak it back and forth in HA and PLL solution 10 times, take it out, use N 2 Blow dry and p...

Embodiment 2

[0028] Add 100ml of deionized water (pH=7.0) to a 250ml small beaker, magnetically stir, slowly add 0.1g of hyaluronic acid to a concentration of 1mg / ml, while stirring, slowly add hydrochloric acid solution to adjust the pH to 2.9 and continue stirring Until it is dissolved, the chitosan solution with pH=2.9 and the polylysine with pH=9.5 are also adjusted, which is the coating liquid, and the corresponding washing liquid with the same pH is adjusted. The glass slide is used as the substrate. The glass slide is clamped with tweezers and immersed in a 5mg / ml PEI solution for 2h for pretreatment. The pretreated glass slides are immersed in HA and CHI for 10 times, each time for 8 minutes. Soak the lotion for 1~2s, dry it with N2, then soak it back and forth in HA and PLL solution 10 times, take it out, and use N 2 Blow dry and place on a glass plate. Dry at room temperature for 24h.

[0029] The static contact angle test film layer showed a certain degree of hydrophobicity, which...

Embodiment 3

[0031] Add 100ml of super-ionized water (pH=7.0) to a 250ml small beaker, magnetically stir, slowly add 0.1g of hyaluronic acid with a concentration of 1mg / ml, while stirring, slowly add hydrochloric acid solution to adjust the pH to 2.9 and continue stirring until it is dissolved. Also adjust the chitosan solution with pH=2.9 and polylysine with pH=9.5, which is the coating liquid. Glass is used as the substrate. The coating method adopts the spin coating method, first spin coating at 1000rad / min for 10s, then spin coating at 2500rad / min for 2min, repeat the operation, and spin coating 5 times. Finally, it was dried at room temperature for 24 hours. The thickness of the film tested by the cross-sectional field emission scanning electron microscope is 0.85±0.18μm. The static contact angle test film showed a certain degree of hydrophobicity, which was 95°±1.32°. Atomic force microscope observation revealed that the surface had very low roughness, and the RMS was 2.44±0.26 nm. ...

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PUM

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Abstract

The invention provides an enzyme-responsive intelligent bacterial adhesion resistant and bactericidal layer-by-layer self-assembled multi-layer film coating and a preparation method thereof. A surface-hydrophobic gel coating which is stable in a water environment is obtained through physical coating of chitosan, hyaluronic acid and a lysine solution, so that a material is endowed with bacterial adhesion resisting and contact sterilizing functions; polylysine improves the bacterial adhesion resistance of the material, so that the coating has the favorable gram-positive and gram-negative bacterial adhesion resisting and sterilizing capabilities. The preparation method is simple in process, quick, mild in condition, and easy for dip-coating, spin-coating, spray-coating and layer-by-layer self-assembly, can be implemented industrially, and has a wide application range, and the antibacterial performance, the bio-compatibility and the lubricity of the surface of medical devices can be effectively improved.

Description

Technical field [0001] The invention specifically relates to the technical field of new materials, in particular to an enzyme-responsive intelligent anti-bacterial adhesion and sterilization layer-by-layer self-assembled multilayer film coating and a preparation method thereof. Background technique [0002] With the rapid development of materials science, medicine, and biology, the research of biomedical materials has made great progress, but it still faces various challenges, and some fundamental problems have not been solved. Among them, in addition to the problem of biocompatibility, biological The main obstacle to material application is infection. In the world, about 64% of hospital-acquired infections are caused by pathogenic bacteria attached to implants or medical devices. In the United States alone, 100,000 people die from related infections every year. In Europe, people spend about 50 million euros a year for the treatment of medical catheter infections, and there are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/16A61L31/14A61L31/10
CPCA61L31/10A61L31/14A61L31/145A61L31/16A61L2300/404A61L2300/424A61L2300/606A61L2400/10A61L2420/08C08L5/08C08L77/04
Inventor 王佰亮南开辉陈浩叶子徐青文刘慧华
Owner WENZHOU MEDICAL UNIV
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