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Carbon dots (CNDs) applicable to detection of dopamine and Al<3+>, cell imaging and logic gate operation

A technology of dopamine, carbon dots, applied in the field of chemistry

Inactive Publication Date: 2017-06-16
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these methods are only able to detect one substance
Published research on the detection of dopamine and Al 3+ This fluorescent probe has almost no

Method used

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  • Carbon dots (CNDs) applicable to detection of dopamine and Al&lt;3+&gt;, cell imaging and logic gate operation
  • Carbon dots (CNDs) applicable to detection of dopamine and Al&lt;3+&gt;, cell imaging and logic gate operation
  • Carbon dots (CNDs) applicable to detection of dopamine and Al&lt;3+&gt;, cell imaging and logic gate operation

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Experimental program
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Effect test

Embodiment 1

[0020] Synthesis of carbon dots (CNDs) of the present invention: 1.92g of anhydrous citric acid and 6.81g of 4,7,10-trioxy-1,13-tridecanediamine were dissolved in 20ml of distilled water, then transferred to 50ml of high pressure Kettle, heated at 200 ° C for 5h. When the reactor was cooled to room temperature we obtained a transparent brown solution, after which we added sodium hydroxide to adjust the pH of the above solution to 14. Rotary evaporation was then performed to obtain a solid, which was then dissolved in ethanol, and the insoluble matter was removed by centrifugation, leaving the supernatant. Finally, the supernatant was further dialyzed in distilled water from a dialysis bag (Mw=3000) for 4 days. Finally, pure carbon dots (CNDs) will be obtained by rotary evaporation.

[0021] Characterization of the carbon dots (CNDs) of the present invention: The structure of the carbon dots (CNDs) was obtained using a transmission electron microscope (TEM). figure 1 a repre...

Embodiment 2

[0024] The carbon dots (CNDs) used in the present invention have sensitivity, selectivity and concentration detection of dopamine.

[0025] (a) Dopamine was gradually added to the carbon dot (CNDs) solution according to the concentration from 0 μM to 200 μM, and the change of fluorescence intensity and the linearity between different dopamine concentrations (20 μM to 80 μM) and fluorescence quenching efficiency F0 / F-1 were detected relation. The results showed that with the addition of dopamine, the fluorescence intensity gradually decreased, indicating that the carbon dots (CNDs) were very sensitive to dopamine and had a very low detection limit of 0.0078 μM.

[0026] (b) Comparison of the effects of different substances on fluorescence quenching. A. Blank sample B. Catechol C. Hydroquinone D. Resorcinol E. Biphenol F. 5-methyl-3-benzenediol G. Dopamine. Tests show that the carbon dots (CNDs) used in the present invention have good selectivity to dopamine.

Embodiment 3

[0028] The carbon dots (CNDs) used in the present invention are to Al 3+ Detection of sensitivity, selectivity and concentration.

[0029] (a) Gradually adding Al to the carbon dots (CNDs) solution containing 1 mM dopamine 3+ (Concentrations from 0 μM to 200 μM) observed a significant increase in fluorescence intensity, indicating that the carbon dots (CNDs)-dopamine system has a strong effect on Al 3+ Sensitivity is good.

[0030] (b) Fluorescence quenching efficiency and Al 3+ Concentration linear relationship, where Al 3+ Concentration range from 4μM to 40μM has a good linear relationship. It was calculated to have a lower detection limit of 0.0329 μM.

[0031] (c) Effect of dopamine-free carbon dot solution on Al 3+ sensitivity test. The results show that dopamine-free carbon dots are beneficial to Al 3+ The sensitivity is very poor, and the fluorescence intensity changes very little.

[0032] (d) Carbon dots to Al 3+ selectivity test. Changes in fluorescence in...

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Abstract

The invention discloses fluorescent CNDs prepared by using a simple one-step hydrothermal method and having quantum yield of 9.9%. The CNDs are applicable as an effective fluorescent probe for sensitive and selective detection of dopamine and Al<3+> ions, with detection limits being 0.78 [mu]M and 3.29 [mu]M, respectively; and the fluorescent probe presents good water solubility, high optical stability, low biotoxicity and other advantages. The fluorescent probe is applicable to logic gate experiments of various molecular levels and will present better adaptability in future logic gate experiments. In particular, the novel fluorescent probe is successively applied to detection of dopamine and Al<3+> ions in real samples. Experimental results in the invention prove that the novel CNDs have great potential in portable sensing devices.

Description

[0001] Technical field: [0002] The invention belongs to the field of chemistry by using fluorescent carbon nano-dots to treat dopamine and Al 3+ applications of detection and logic gate manipulation. [0003] Background technique: [0004] Dopamine is an important neurotransmitter in the brain, released by nerve cells that then transmit signals to other nerve cells. But abnormal levels of dopamine in the brain have been linked to diseases such as Parkinson's and Alzheimer's. On the other hand, like Al 3+ Such metal ions play a vital role in many life activities, such as trace elements in the human body. Many studies have shown that excess Al 3+ Ingestion can cause kidney failure, osteoporosis, and neurological disorders. Therefore, detection of dopamine and Al 3+ It is critical in controlling concentrations in living organisms and environmental systems. So far, various methods including liquid chromatography, chemiluminescence, capillary electrophoresis, and fluorescen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 颜范勇王银银祖凡淋张金贠凯祎刘世起陈莉
Owner TIANJIN POLYTECHNIC UNIV
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