A kind of biocontrol strain ls01 and its biocontrol agent for preventing and treating asparagus stem blight
A technology for asparagus stem blight and biocontrol fungicides, applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of high pesticide residues, high costs, outstanding chemical pesticide resistance, etc., and achieve good control effects, The effect of not polluting the environment and not being resistant to drug resistance
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Embodiment 1
[0011] Example 1 Paenibacillus polymyxa LS01 biocontrol agent and its preparation method.
[0012] In this example, Paenibacillus polymyxa LS01 (Paenibacillus polymyxa CGMCC NO. 11690) is used to make a biocontrol agent. Obtained after the bacterial agent is prepared, the specific preparation method comprises the following steps:
[0013] (1) Activation: Inoculate Paenibacillus polymyxa LS01 onto LB solid medium at a culture temperature of 37°C for 3-4 days; then transfer to LB liquid medium at a temperature of 30°C and shake at 180 rpm Cultivate for 16-28h.
[0014] (2) Seed culture: transfer the activated bacterial solution in step (1) into a 250 mL Erlenmeyer flask, at 30°C, 180 rpm, and vibrate for 16-28 hours until the logarithmic growth phase;
[0015] (3) Production culture: inoculate the bacterial liquid obtained in step (2) into the fermenter at an inoculation amount of 0.5%-2% of the total volume of the LB production medium, the tank temperature is 30°C, and the st...
Embodiment 2
[0017] Example 2 The inhibitory effect of Paenibacillus polymyxa LS01 biocontrol agent on Phomopsis asparagus.
[0018] Phomopsis, the pathogen of asparagus stem blight, has been activated twice, add 10mL of sterile water, carefully scrape off the spores and hyphae with an inoculation needle, then pour it into a triangular flask with glass beads, and shake it on a shaker Break up the bacteria block and let it stand still. Pour the supernatant into 300mL PDA medium that has been cooled to 45°C. After shaking well, pour it onto the plate immediately. After cooling and solidifying, place sterile small round filter paper (2 pieces / dish) , and cultured overnight at 30°C. Dilute the Paenibacillus polymyxa LS01 biocontrol agent and sterile water at a ratio of 1:20, then take 3 μL dropwise on the filter paper of the pathogen culture medium, use sterile water as the blank control group, and incubate at 30°C for 24-36 h , observe the inhibition zone.
[0019] The results of the antiba...
Embodiment 3
[0022] Example 3 Greenhouse pot test to detect the control effect of Paenibacillus polymyxa LS01 biocontrol agent on asparagus stem blight
[0023] Before transplanting, after diluting 100 times with Paenibacillus polymyxa LS01 biocontrol agent, soak the asparagus seedlings for 30 minutes; when transplanting, use 20 mL of biocontrol agent dilution to irrigate the roots, and inoculate 1000 / mL Phomopsis asparagus zoospore suspension 20mL. In addition, the treatment of directly inoculating the zoospore suspension of Phomopsis asparagus without Paenibacillus polymyxa LS01 biocontrol agent was used as the control group. Each treatment group consisted of 20 plants, repeated three times. At a temperature of 25-28°C and a relative humidity of 50%, the disease progression was investigated after 4 weeks of cultivation, and the disease severity and control effect were calculated.
[0024] The grading standard of root irrigation and inoculation method:
[0025] Grade 0, without any s...
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