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Method for improving desorption ionization efficiency by enriching analyte to metal nanocone array tip

A metal nanometer and analyte technology, applied in the field of detection, can solve the problems that are not conducive to improving the desorption and ionization efficiency of the analyte molecules, the energy utilization efficiency is not high, and the laser energy needs to be further improved.

Inactive Publication Date: 2017-06-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the random distribution of analyte molecules, the utilization efficiency of laser energy still needs to be further improved.
[0004] Therefore, the current surface-assisted laser desorption ionization mass spectrometry substrate designed based on silicon materials has the problem of low energy utilization efficiency, which is not conducive to improving the desorption ionization efficiency of analyte molecules.

Method used

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  • Method for improving desorption ionization efficiency by enriching analyte to metal nanocone array tip
  • Method for improving desorption ionization efficiency by enriching analyte to metal nanocone array tip
  • Method for improving desorption ionization efficiency by enriching analyte to metal nanocone array tip

Examples

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Effect test

Embodiment 1

[0062] First cut the silicon wafer into 10 pieces, of which 9 pieces have a size of 1.5cm×1.5cm, and one piece has a size of 4cm×6cm, and then use acetone, chloroform, ethanol, and deionized water to ultrasonically clean each at 70W for 5 minutes. Prepare NH 3 ·H 2 O (25% aqueous solution by mass fraction): H 2 o 2 (30% aqueous solution by mass fraction): H 2 O=1:1:5 (volume ratio) mixed solution, heat it to 90°C, then immerse the cleaned silicon chip in the solution and cook for 40min. Finally, they were rinsed with deionized water and dried with nitrogen to obtain ball-raising silicon wafers (1.5 cm×1.5 cm) and volleyball silicon wafers (4 cm×6 cm).

[0063] Add 0.1 g of polystyrene microsphere powder with a diameter of 600 nm into 2 mL of a mixed solution of deionized water and ethanol at a volume ratio of 1:1, ultrasonicate for 6 hours, and mix it evenly to obtain ethanol and ethanol of polystyrene microspheres. Deionized water mixed solution. Add deionized water to ...

Embodiment 2

[0068] 2 μL of water was added dropwise to the gold film-covered silicon nanocone array and the perfluorothiol-modified gold-covered silicon nanocone array obtained in different steps of Example 1 to measure the contact angle and rolling angle.

Embodiment 3

[0070] Put the gold-covered silicon nanocone arrays and perfluorothiol-modified gold-covered silicon nanocone arrays obtained in different steps in Example 1 into the instrument for spectral testing. This test was performed in Shanghai Fuxiang R1-A-UV series Tested on a spectrometer.

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Abstract

The invention discloses a surface assistant laser desorption ionization mass spectrometry analysis method for improving desorption ionization efficiency of analyte by enriching the analyte to a metal nanocone array tip, and belongs to the technical field of detection. A metal membrane is applied to the surface of a silicon nanocone array in an evaporation manner, then surface plasma element conduction is carried out, photon energy is accumulated in tip positions, and thus a photon library is formed. As the surface is modified with fluoro-substitution alkyl sulfhydryl, a substrate has a large contact angle and a small rolling angle, then the friction of water droplets on the substrate is very small, analyte molecules of a solution are preferentially concentrated at the tips, and the utilization rate of laser energy can be further increased. Therefore, when the silicon nanocone array covered by the metal membrane modified with the fluoro-substitution alkyl sulfhydryl is used as substrate detection analyte molecules, the laser energy can be sufficiently absorbed and effectively utilized, furthermore the desorption ionization efficiency of the analyte molecules can be improved, and the method is applicable to various types of molecules.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a surface-assisted laser desorption ionization mass spectrometry method for improving the desorption and ionization efficiency of the analyte by enriching the analyte on the tip of the metal nanocone array. Background technique [0002] Surface-assisted laser desorption ionization mass spectrometry does not need to add organic matrix during the detection process, so there is no impurity interference in the low molecular weight region, which is very suitable for testing small molecules. For surface-assisted laser desorption ionization mass spectrometry, the performance of the substrate determines the analytical ionization efficiency of the analyte, so it is very important to design a substrate that can absorb and utilize laser energy efficiently. In the field of surface-assisted laser desorption ionization mass spectrometry, the substrates used can be roughly divided...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N1/40
CPCG01N1/40G01N27/64
Inventor 吕男李宁窦树珍滕飞杜娟
Owner JILIN UNIV
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