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Preparation method and applications of fungus chaff compost inoculum

A technology of bacterial agent and compost, which is applied in the field of preparation of compost bacterial agent, can solve problems such as difficulty in achieving stable effect and expected effect, difficulty in ensuring the proliferation of member bacteria, and prolonging the compost maturity time, so as to save energy and simplify the manufacturing process , Decomposition function stabilization effect

Inactive Publication Date: 2017-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are: ① Some bacterial agents pay too much attention to the heating effect, and choose mesophilic microorganisms for easily decomposed substances as the microbial agent. Since they do not have the ability to decompose the key component lignocellulose, the high temperature period disappears rapidly, and the desired effect cannot be achieved; ② Excessive emphasis on aerobic conditions promotes the easy-to-decompose starch, fat, protein and other components, but inhibits the decomposition of refractory lignocellulose, resulting in the need for a longer non-ventilated secondary fermentation to decompose the refractory, thus prolonging Compost maturity time; ③ The bacterial agents used in the compost fermentation environment, the constituent bacteria are all in the same environment, it is very important whether these member bacteria can grow simultaneously in the same environment and whether their functions can cooperate with each other
As a result, it is often difficult to ensure that these member bacteria proliferate well in the same environment, and it is even more difficult to achieve stable and expected effects.

Method used

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  • Preparation method and applications of fungus chaff compost inoculum
  • Preparation method and applications of fungus chaff compost inoculum
  • Preparation method and applications of fungus chaff compost inoculum

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Embodiment Construction

[0031]The present invention will be further elaborated and illustrated below in conjunction with the accompanying drawings and specific embodiments. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0032] 1. Experimental materials

[0033] 1. The strains required for the preparation of bacterial agents

[0034] No. 1 strain: Bacillus genus GX-5, the strain is Bacillus subtilis; No. 2 strain: Brevibacillus genus GX-7, the strain is Brevibacillus pyrodorus; No. 3 strain: Brevibacillus genus GX-13 , the strain is Brevibacillus reuteri; No. 4 strain: Brevibacillus genus GX-16, the strain is Brevibacillus lateralsporosa.

[0035] 2. The culture medium required for the preparation of bacterial agents

[0036] Medium A: The solvent is water, and the solute and concentration are as follows: CMC-N...

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Abstract

The invention discloses a preparation method and applications of a fungus chaff compost inoculum. The compost inoculum provided by the invention contains the active ingredients including bacillus subtilis, brevibacillus thermoruber, brevibacillus reuszeri and brevibacillus laterosporus at the CFU ratio being (1-2) to (1-2) to (1-2). For the inoculum provided by the invention, the bacterial strains rapidly proliferate together under the micro-oxygen condition, so that lignocellulose is efficiently decomposed. For the inoculum, the preparation process is simple, the decomposition function is stable, and the expiration date is long. In the embodiment, for an inoculum group in which the inoculum is fed, compared with a control group in which the inoculum is not added, the composting time is shortened by 6 days, the degradation rates of cellulose, hemicellulose and lignin are improved by 5.69%, 6.70% and 4.57% respectively, the loss ratio of total nitrogen is reduced by 9.44%, the content of humus is improved by 13.51%, and the germination index of seeds is improved by 8%. The experiment proves that when the inoculum is adopted for fermenting fungus chaff compost, the abundant ventilation is not needed, therefore, energies are saved, meanwhile, the nitrogen loss can be reduced, the compost maturity can be promoted, and the compost quality can be improved.

Description

technical field [0001] The invention relates to a preparation method and application of a composting bacterial agent, in particular to a high-temperature composting compound bacterial agent of bacteria chaff and its preparation method and application. Background technique [0002] With the development of the economy and the continuous improvement of people's requirements for food types and quality, the demand for edible fungi is increasing day by day, and the output is also increasing. Yet along with the development of edible fungus industry, the quantity of discarded solid culture medium, i.e. fungus chaff, after harvesting the fruiting bodies of edible fungi is also increasing. According to investigations, about 5.0 kg of fungus chaff is produced for every 1.0 kg of edible fungus produced (Paredes et al., 2009). [0003] Most of the fungus chaff is discarded at will, causing environmental pollution. Rational development and utilization of edible fungus chaff to make effi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/125C12R1/01
CPCC05F5/00C05F17/00C12N1/20C05F11/08Y02W30/40
Inventor 胡宝兰诸葛诚祥潘亚威梁程钧楼莉萍
Owner ZHEJIANG UNIV
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