Preparation method and application of spirulina whole active substances
A technology for active substances and spirulina, which is applied in the field of preparation of all active substances of spirulina, can solve the problems of low utilization rate and incomplete preparation of active substances, and achieves the effects of high repeatability, strong practicability, and stable and reliable process.
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Embodiment 1
[0026] A method for preparing the active substance of spirulina, using spirulina as raw material, using solvent extraction with different polarities (from small to large) and enzymatic hydrolysis with compound protease to prepare the active substance of spirulina:
[0027] First extract with 85wt% ethanol, then extract the residue of 85wt% ethanol extraction with 65wt% ethanol, then extract the residue of 65wt% ethanol with water, and finally enzymolyze the water extraction residue, and combine the above alcohol extraction, water extraction and enzymolysis The obtained extract was centrifuged, filtered, decompressed, concentrated and dried to obtain 85wt% ethanol extract of spirulina, 65wt% ethanol extract of spirulina, water extract of spirulina and enzymatic hydrolyzate of spirulina, namely: spirulina Full active substance; Concretely comprises the following steps:
[0028] A. Spirulina fine powder was first extracted with 85wt% ethanol at 45°C for 3 hours by ultrasonic-micr...
Embodiment 2
[0035] A method for preparing the active substance of spirulina, using spirulina as raw material, using solvent extraction with different polarities (from small to large) and enzymatic hydrolysis with compound protease to prepare the active substance of spirulina:
[0036] First extract with 95wt% ethanol, then extract the residue of 95wt% ethanol extraction with 55wt% ethanol, then extract the residue of 55wt% ethanol with water, and finally enzymolyze the residue of water extraction, the above alcohol extraction, water extraction and enzymolysis The obtained extracts were centrifuged, filtered, decompressed, concentrated and dried to obtain Spirulina 95wt% ethanol extract, Spirulina 55wt% ethanol extract, Spirulina water extract and Spirulina enzymatic hydrolyzate, namely: Spirulina Full active substance; Concretely comprises the following steps:
[0037] A. Spirulina fine powder was extracted with 95wt% ethanol at 70°C for 0.5h by ultrasonic-microwave co-extraction, and fil...
Embodiment 3
[0044] A method for preparing the whole active substance of spirulina, using spirulina as raw material, using solvents of different polarities (from small to large) to extract and prepare the whole active substance of spirulina:
[0045] First extract with 90wt% ethanol, then extract the residue of 90wt% ethanol extraction with 60wt% ethanol, then extract the residue of 60wt% ethanol with water, and finally enzymolyze the water extraction residue, and combine the above alcohol extraction, water extraction and enzymolysis The extract obtained is centrifuged, filtered, decompressed, concentrated and dried to obtain 90wt% ethanol extract of spirulina, 60wt% ethanol extract of spirulina, water extract of spirulina and enzymatic hydrolyzate of spirulina, namely: spirulina Full active substance; Concretely comprises the following steps:
[0046] A. Use 90wt% ethanol to extract spirulina fine powder at 55°C for 1.5h with ultrasonic assistance, and filter to obtain 90wt% ethanol extra...
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