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Medicine composition containing compound 20-(R)-notoginsenoside Ft1 and application of medicine composition in pharmacy

A technology of notoginseng saponins and compounds, applied in the field of medicine, can solve the problems of undiscovered compound application, undiscovered and the like

Inactive Publication Date: 2017-07-07
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the application and report of 20-(R)-notoginsenoside Ft1 (20-(R)-notoginsenoside Ft1) has not been found as an active ingredient in anti-tumor and improving immune function, nor has it been found that the compound is used in the preparation of anti-tumor and improving immune function. Reports on the Application of Immune Function Drugs and Functional Foods

Method used

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  • Medicine composition containing compound 20-(R)-notoginsenoside Ft1 and application of medicine composition in pharmacy
  • Medicine composition containing compound 20-(R)-notoginsenoside Ft1 and application of medicine composition in pharmacy
  • Medicine composition containing compound 20-(R)-notoginsenoside Ft1 and application of medicine composition in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of 20-(R)-notoginsenoside Ft1 (20-(R)-notoginsenoside Ft1):

[0030] The stems and leaves of notoginseng are extracted with 10 times the amount of 1% HCl solution, extracted twice, each time for 2 hours, the filtrates are combined, the pH is adjusted to 7 with 3% NaOH solution, concentrated and dried to obtain a dry paste. Dry paste 100g, mix the sample with silica gel (200-300 mesh) and carry out silica gel column chromatography, use chloroform: methanol: water (8:2:0.2) as eluent, thin layer chromatography checks and merges the same color point Fractions, Fr1-5 fractions were obtained. Fr4 (5g) is carried out repeatedly silica gel column chromatography with chloroform: methanol: water (8:2:0.2) as eluent, and reverse phase silica gel RP-18 column chromatography (methanol: water / 8:2), obtains 20 -(R)-Notoginsenoside Ft1 (0.16 g).

[0031] Structural identification of 20-(R)-notoginsenoside Ft1 (20-(R)-notoginsenoside Ft1):

[0032] Instrument: mass spect...

Embodiment 2

[0039] In vivo anti-tumor activity experiment of 20-(R)-notoginsenoside Ft1 (20-(R)-notoginsenosideFt1):

[0040] 1. Drugs

[0041] (1) Test substance

[0042] 20-(R)-Notoginsenoside Ft1 was fully ground in a mortar, and prepared with 0.5% sodium carboxymethylcellulose to make solutions with final concentrations of 120 mg / kg, 80 mg / kg, 40 mg / kg, and 20 mg / kg. Ready to use daily.

[0043] (2) Positive control drug

[0044] Cyclophosphamide for injection, approved by the Chinese National Medicines Agency H32020857, Jiangsu Shengdi Pharmaceutical Co., Ltd. Dilute to 2mg / mL with normal saline during the experiment.

[0045] 2. Animals

[0046] (1) Strain and source

[0047] SPF grade ICR mice, female, weighing 20-22 g, were provided by the Experimental Animal Center of Kunming Medical University, production license number: SCXK (Dian) K2015-0002, license issuing authority: Kunming Science and Technology Bureau. Adaptive feeding 5 days into the experiment.

[0048] (2) Feed...

Embodiment 3

[0065] 20-(R)-Notoginsenoside Ft1 (NotoginsenosideFt1) in vitro immune activity test:

[0066] 1. Test drug

[0067] 20-(R)-notoginsenoside Ft1, dissolved in DMSO to prepare 40mmol / L stock solution, stored at -20°C, and diluted with RPMI-1640 medium to final concentrations of 10umol / L, 5umol / L, 2.5umol in experiments / L, 1.25umol / L, 0.625umol / L, 0.3875umol / L.

[0068] 2. Main instruments

[0069] MS104S / PL600-S electronic analytical balance, METTLER TOLEDO, Switzerland; QT-1 vortex mixer, Shanghai Qite Instrument Analysis Instrument Co., Ltd.; carbon dioxide incubator, American Thermo-fisher Epoch continuous wavelength microplate reader, American Bio-Tek; Micropipettes, eppendorf.

[0070] 3. Main reagents

[0071] RPMI-1640 medium was purchased from BI Company (Cat. No. 0011616); fetal bovine serum was purchased from BI Company in the United States, Concanavalin A (ConcanavalinA, ConA, Cat. No.: C2272), bacterial lipopolysaccharide (LPS, Cat. No.: L2630), MTT All were pu...

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Abstract

The invention provides a medicine compositionforresisting a tumor and improving an immunologic function of a body. The medicine composition takes a compound, namely 20-(R)-notoginsenoside Ft1 as an active ingredient, and comprises a conventional pharmaceutical carrier.At the same time, the invention provides application of the compound 20-(R)-notoginsenoside Ft1 in preparing a medicine for resisting the tumor and improving the immunologic function of the body, and application of the compound 20-(R)-notoginsenoside Ft1 in preparing functional food for resisting the tumor and improving the immunologic function of the body.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of medicines, and specifically relates to the application of compound 20-(R)-notoginsenoside Ft1 (20-(R)-notoginsenoside Ft1) in anti-tumor and immune-improving medicines and functional foods. [0003] Background technique: [0004] In China, cancer has become the number one cause of death, and the morbidity and mortality are still rising, posing a huge threat to public health. According to the 2015 annual report released by the National Cancer Registry Center, there are about 3.12 million new cancer cases in my country every year, an average of 8,550 people are diagnosed every day, 6 people are diagnosed with cancer every minute, and one person is diagnosed with cancer every 10 seconds. Diagnosed. According to statistics, more than 2.8 million people died of cancer in China in 2015, an average of 7,500 people per day. The World Health Organization (WHO) released the "World Cancer Report" before the ...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P35/00A61P37/04A23L33/105
CPCA61K31/704A23V2002/00A23V2200/308A23V2200/324A23V2250/21
Inventor 沈勇李燕顺
Owner YUNNAN AGRICULTURAL UNIVERSITY