Electrically active ta-ha composite nerve guide and preparation method thereof

A TA-HA, nerve catheter technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of sacrificing secondary nerve function, clinical application limitations, limited nerve sources, etc., to achieve good biocompatibility, reduce Toxic and side effects, good biocompatibility

Active Publication Date: 2020-06-19
深圳南泥湾科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, autologous nerve transplantation has the disadvantages of limited nerve source, sacrificing secondary nerve function, producing donor site neuroma, scar formation, and infection, and its clinical application is limited.

Method used

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  • Electrically active ta-ha composite nerve guide and preparation method thereof
  • Electrically active ta-ha composite nerve guide and preparation method thereof
  • Electrically active ta-ha composite nerve guide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Formylated HAALD of hyaluronic acid:

[0026] Dissolve 0.1g of hyaluronic acid in 50mL of deionized water, adjust the pH between 5-6, dissolve under magnetic stirring, add 10ml of 0.25mol / L sodium periodate, react at room temperature for 4-5h, add 10ml of ethylene glycol Alcohol terminated the reaction, dialyzed for three days, changed the water, removed unreacted substances, and obtained powdered formylated hyaluronic acid after freeze-drying.

[0027] 2) Preparation of TA:

[0028] Dissolve 0.5g of N-phenyl-p-phenylenediamine in a precooled mixed solvent of 1mol / L HCl and acetone, stir magnetically at 0°C, dissolve 0.5g of ammonium persulfate in a mixed solvent of acetone and water, and Ammonium sulfate was slowly added dropwise to the N-phenyl-p-phenylenediamine system, stirred magnetically for 3 h, washed and filtered, counter-doped with 0.1 mol / L ammonia water, then reduced with 5 ml of phenylhydrazine, filtered, and vacuum-dried to obtain TA powder.

[0029] ...

Embodiment 2

[0039] 1) Formylated HAALD of hyaluronic acid:

[0040] Dissolve 0.1g of hyaluronic acid in 50mL of deionized water, adjust the pH between 5-6, dissolve under magnetic stirring, add 15ml of 0.25mol / L sodium periodate, react at room temperature for 4-5h, add 20ml of ethylene glycol Alcohol terminated the reaction, dialyzed for three days, changed the water, removed unreacted substances, and obtained powdered formylated hyaluronic acid after freeze-drying.

[0041] 2) Preparation of TA:

[0042] Dissolve 0.5g of N-phenyl-p-phenylenediamine in a precooled mixed solvent of 1.5mol / L HCl and acetone, stir magnetically at 0°C, dissolve 0.8g of ammonium persulfate in a mixed solvent of acetone and water, and Ammonium sulfate was slowly added dropwise to the N-phenyl-p-phenylenediamine system, stirred magnetically for 3 hours, washed and filtered, counter-doped with 0.15mol / L ammonia water, then reduced with 5ml of phenylhydrazine, filtered, and vacuum-dried to obtain TA powder.

[0...

Embodiment 3

[0048] 1) Formylated HAALD of hyaluronic acid:

[0049] Dissolve 0.2g of hyaluronic acid in 50mL of deionized water, adjust the pH between 5-6, dissolve under magnetic stirring, add 10ml of 0.25mol / L sodium periodate, react at room temperature for 4-5h, add 10ml of ethylene glycol Alcohol terminated the reaction, dialyzed for three days, changed the water, removed unreacted substances, and obtained powdered formylated hyaluronic acid after freeze-drying.

[0050] 2) Preparation of TA:

[0051] Dissolve 0.5g of N-phenyl-p-phenylenediamine in a precooled mixed solvent of 1mol / L HCl and acetone, stir magnetically at 0°C, dissolve 0.5g of ammonium persulfate in a mixed solvent of acetone and water, and dissolve the persulfuric acid Ammonium was slowly added dropwise to the N-phenyl-p-phenylenediamine system, stirred magnetically for 5 hours, washed and filtered, counter-doped with 0.1mol / L ammonia water, then reduced with 10ml of phenylhydrazine, filtered, and vacuum-dried to obt...

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Abstract

The invention relates to a TA-HA compound nerve conduit with electroactivity and a preparation method thereof. The preparation method of the TA-HA compound nerve conduit comprises the following steps: (1) dissolving TA powder, adding into formylated hyaluronic acid HAALD, stirring, filtering, pouring into precooled ethanol alcohol, stirring, centrifuging, performing suction filtration, and drying to obtain TA-HAALD; (2) dissolving in ethyl acetate, performing ultrasonic treatment, adding NGF lyophilized powder into a suspension, performing ultrasonic dispersion, adding PDLLA, stirring and dissolving to completely disperse each component in the solution, pouring the mixed solution into a mold, evaporating at room temperature to form a film, taking off after the film is completely dried, preparing a tube by the film with a mold, drying in vacuum to constant weight, and sterilizing with ultraviolet radiation. The TA-HA compound nerve conduit has the beneficial effect of meeting the electrical stimulation condition essential to nerve growth along with good biocompatibility necessary to a traditional conduit, and the mechanical strength of the TA-HA compound nerve conduit can meet the physiological environment of the conduit for supporting the nerve broken position.

Description

technical field [0001] The invention relates to a nerve guide biomaterial, in particular to a TA-HA composite nerve guide with electrical activity and a preparation method thereof. Background technique [0002] More than 1,000,000 people around the world suffer from peripheral nerve damage every year, mostly caused by transverse injuries, burns, or degenerative diseases. Due to the complex pathological process after peripheral nerve damage, nerve regeneration is slow, regenerative nerves adhere to surrounding tissues, and neuromuscular atrophy Many factors, such as motor end plate degeneration and degeneration, restrict the functional recovery of injured nerves. Small nerve defects can be automatically repaired through the inherent nerve regeneration ability, while the repair and functional reconstruction of long peripheral nerve defects is still one of the clinical problems in hand surgery and microsurgery, which seriously affects the quality of life of patients. At presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/58A61L27/56A61L27/54A61L27/50A61L27/20A61L27/18
CPCA61L27/18A61L27/20A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/252A61L2300/414A61L2430/32C08L5/08C08L67/04
Inventor 徐海星郭兴蕾许沛虎黄志军马慧布颖张希郑芙蓉向梅
Owner 深圳南泥湾科技有限公司
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