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Bacillus licheniformis strain BL06 and application thereof

A technology of Bacillus licheniformis and strains, applied in the field of agricultural microorganisms, to achieve the effect of reducing residue problems and improving quality

Active Publication Date: 2017-07-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, consumers are gradually aware of the impact of chemical pesticide residues on human health, and all walks of life have turned to use biological pesticides, such as Kejunkang, Wuyimectin, Ludunfeng, and Shuangbang. Bacillus and Pseudomonas, but mostly in laboratory development

Method used

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  • Bacillus licheniformis strain BL06 and application thereof
  • Bacillus licheniformis strain BL06 and application thereof
  • Bacillus licheniformis strain BL06 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening method: Bacillus licheniformis BL06 (CGMCC NO.13462) was isolated from the cucumber rhizosphere in Dingji Town, Huaian City, Jiangsu Province. Cut the cucumber root tissue into small pieces of 1 cm, soak in 1% sodium hypochlorite for 5 minutes, soak in 70% alcohol for 1-2 minutes, and wash with sterile water for 3 times (take the last cleaning solution 100 μl and apply it to R 2 A solid culture medium, if sterile growth after 48h, it is considered that the surface is clean and sterile). Take 3g of the sterilized sample and place it in a sterile mortar, add 27ml of sterile 0.85% NaCl, macerate the tissue, grind it, and dilute with sterile 0.85% NaCl gradient. take 10 -1 、10 -2 、10 -3 100 μl each of the three gradient dilutions were applied to R 2 On A, culture at 28°C for 48h. Pick the largest single colony to inoculate into fresh R 2 A solid medium, through repeated transfer to obtain purified strains. The purified strains were stored in -70°C ultra-low...

Embodiment 2

[0032] The preparation of embodiment 2BL06 biocontrol agent

[0033] Inoculate the Bacillus licheniformis BL06 (CGMCC NO.13462) strain into the LB culture medium for 16 hours at 28°C with shaking at 200rpm, then take a sample every 2 hours in the ultra-clean workbench to measure its OD value at 600nm, when the OD value is 0.8 When the culture is over, this bacterial solution is used as the seed bacterial solution. Inoculate the seed bacteria solution into LB fermentation medium at a volume ratio of 1:500 for fermentation and culture, culture at 28°C and 200 rpm for 48 hours, then centrifuge at 6000 rpm for 10 minutes, and collect the precipitate to obtain the biocontrol agent HS10. The total concentration of viable bacteria in the resulting biocontrol agent was 1×10 9 ~1×10 10 CFU / mL.

Embodiment 3

[0034] Example 3 Greenhouse Experiment Determination of BL06's Growth-promoting Effect on Cucumber

[0035] The biocontrol bacteria treatment group used 30ml concentration of 10 for each seedling 8 The bacterial solution of CFU / ml was treated, and the control group was treated with clear water. Each treatment had 12 seedlings, and each treatment was repeated 3 times. 25 days after inoculation, the cucumber plant height, leaf area, fresh weight and dry weight were measured. Biomass increase (%)=(fresh weight of plants in treatment group-fresh weight of control plants) / fresh weight of control plants×100%.

[0036] The growth promotion data in the greenhouse showed (Table 2) that the cucumber plants treated with BL06 were higher than the control in terms of plant height, leaf area, and fresh weight, and the plant biomass increment was 15.62%. The dry weight of the melon seedlings treated with BL06 was 4.89g / plant, which was significantly smaller than that of the control group, ...

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Abstract

The invention discloses a Bacillus licheniformis strain BL06 and application thereof. The Bacillus licheniformis strain BL06 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 16, 2016, with a strain preservation number No.13462. The Bacillus licheniformis strain BL06 can be used for preparing biological preparations for controlling Brassica soft rot, and promoting plant growth.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to bacillus licheniformis BL06 and application thereof. Background technique [0002] Bacillus licheniformis is a strain within the genus Bacillus. It is one of the strains that have been widely used and researched and has great potential development value. The bacteria can inhibit pathogenic bacteria by producing antibacterial active substances and having a unique mechanism of biological oxygen capture. The growth and reproduction of plants can produce a variety of growth-promoting related substances to promote plant growth. [0003] In agricultural production, plant pathogenic fungal diseases are pathogens that threaten crop production and affect crop yield and quality. Among them, cucumber downy mildew is caused by Pseudoperonospora cubensis, which is one of the most important leaf diseases in cucumber producing areas all over the world. This disease has occurred in mor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/10
CPCA01N63/00C12N1/20C12N1/205C12R2001/10
Inventor 刘红霞徐龙黄文祥
Owner NANJING AGRICULTURAL UNIVERSITY
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