Acinetobacter schindleri MCDA 01 and method for preparing chitin deacetylase from Acinetobacter schindleri MCDA 01

Abstract

The invention discloses Acinetobacter schindleri MCDA 01 from the sea. The Acinetobacter schindleri MCDA 01 was preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.13538. The strain is gram-negative brevibacterium, and has characteristics of no spore, white and nontransparent bacterial colony, smooth and moist surface with slight bulges and serrulateserrulated edge of bacterial colony. According to the strain, optimum growth temperature is 30 DEG C, optimum growth pH is 8.0, optimum growth NaCl concentration is 4%, and optimum fermentation pH of chitin deacetylase produced from the MCDA01 strain is 8.0 and the optimum temperature is 30 DEG C. The prepared chitin deacetylase has high activity at low temperature and helps save energy and cost in industrial application. Chitosan by utilization of enzymatic preparation has high degree of deacetylation, the biological enzyme method is green and environment-friendly, and green production of chitosan is realized. The invention has great economic benefits.

Description

technical field [0001] The invention relates to a microorganism, which is a kind of Acinetobacter tenmomu from the ocean, in particular a kind of Acinetobacter tenmomu MCDA01 and a method for preparing chitin deacetylase thereof. Background technique [0002] Chitin, also known as chitin and chitin, is a substance second only to cellulose in natural organic compounds. It is widely found in the exoskeleton and epidermis of invertebrates and the cell walls of fungi and algae. Chitin is a linear macromolecular substance connected by N-acetylglucosamine through β-1,4 glycosidic bonds. It is insoluble in water and organic solvents, and is not easy to be widely developed and utilized. If the deacetylation degree of chitin exceeds 55%, chitosan can be obtained, and its solubility increases, and it has biological activities such as anti-cancer, lowering cholesterol, and lowering blood pressure. It can be widely used in medicine, food, chemical industry, cosmetics, etc. industry, wi...

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Problems solved by technology

[0004] In order to solve the disadvantages of the above-mentioned chemical hot alkali method, such as not being environmentally friendly, poor product stability, difficult to control, and the biological enzymolysis method, such as low enzyme activity, poor deacetylation effect, and high cost, the present invention provides a green, environmentally friendly, and improved deacetylation method. The method for the degree of acetylation of chitin deacetylase, the specific scheme is as follows:

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