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Self assembled gold nanorod SERS immune base and preparation method thereof

A technology of gold nanorods and self-assembly, which can be used in measurement devices, material excitation analysis, instruments, etc., can solve the problems of limited application scope, easy to be affected by the coffee circle effect, small scale of gold nanoparticle assembly structure, etc., and is easy to popularize. , The effect of enhancing SERS signal intensity and high yield

Active Publication Date: 2017-07-11
东营睿港投资服务有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the patent application number 201310015549.3 in the prior art discloses a method for preparing an immunoactive substrate. Although the preparation process of the method is simple, the assembled structure of the prepared gold nanoparticles is small in size, easily affected by the coffee ring effect, and the scope of application is limited.

Method used

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  • Self assembled gold nanorod SERS immune base and preparation method thereof
  • Self assembled gold nanorod SERS immune base and preparation method thereof
  • Self assembled gold nanorod SERS immune base and preparation method thereof

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preparation example Construction

[0042] The invention provides the preparation method of the self-assembled gold nanorod SERS immune substrate, comprising the following steps:

[0043] 1) Mix 0.45~0.55mmol / L chloroauric acid solution, 0.15~0.25mol / L cetyltrimethylammonium bromide solution, 0.005~0.02 sodium borohydride solution and deionized water according to the volume ratio of 10 ~15: 10~15: 5.0~6.0: 1 mixed and left to stand to obtain gold seed solution;

[0044] 2) Mix 0.0768mol / L and 0.0162mol / L sodium oleate solution according to the volume ratio of 1:1 until completely dissolved, then add 4mmol / L silver nitrate solution and 1mmol / L chloroauric acid solution to the mixed solution in turn, the volume concentration 37% hydrochloric acid solution and 10mmol / L ascorbic acid solution, obtain growth liquid; The 4mmol / L silver nitrate solution that described mixed solution adds successively, 1mmol / L chloroauric acid solution, 37% hydrochloric acid solution and 10mmol / L ascorbic acid solution The volume ratio...

Embodiment 1

[0082] Prepare a monodisperse uniform gold nanorod solution, the specific process is: a1. Stir 5mL of 0.50mM chloroauric acid solution and 5mL of 0.20M hexadecyltrimethylammonium bromide solution at room temperature, and quickly add Add frozen 0.6mL 0.01M sodium borohydride solution, and add 0.4mL deionized water to obtain gold seed solution, stand at room temperature for at least 2h for use; a2, add 2.5mL 0.0768M cetyltrimethyl bromide Mix ammonium chloride and 2.5mL 0.0162M sodium oleate solution until completely dissolved, add 180μL 4mM silver nitrate solution to it, let stand for 15min, then add 2.5mL 1mM chloroauric acid solution, stir for 15min, the solution becomes colorless Finally, add 21 μL of 37% hydrochloric acid solution, and then add 12 μL of 10 mM ascorbic acid solution to obtain a growth solution; a3. Add 8 μL of the gold seed solution prepared in step a1 to the growth solution prepared in step a2, and let it stand at room temperature After 12 hours, the gold n...

Embodiment 2

[0086] Prepare a monodisperse uniform gold nanorod solution, the specific process is: a1. Stir 5mL of 0.45mM chloroauric acid solution and 5mL of 0.25M hexadecyltrimethylammonium bromide solution at room temperature, and quickly add Add frozen 0.6mL 0.005M sodium borohydride solution, and add 0.4mL deionized water to obtain a gold seed solution, and let it stand at room temperature for at least 2 hours; Mix ammonium chloride and 2.5mL 0.0162M sodium oleate solution until completely dissolved, add 180μL 4mM silver nitrate solution to it, let stand for 15min, then add 2.5mL 1mM chloroauric acid solution, stir for 15min, the solution becomes colorless Finally, add 21 μL of 37% hydrochloric acid solution, and then add 12 μL of 10 mM ascorbic acid solution to obtain a growth solution; a3. Add 8 μL of the gold seed solution prepared in step a1 to the growth solution prepared in step a2, and let it stand at room temperature After 12 hours, the gold nanorod sol was finally obtained. ...

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Abstract

The invention provides a preparation method of a controllable self-assembly gold nanorod SERS immune base. The SERS immune base includes a single-side polished silicon wafer, gold nanorods fixed on the silicon wafer polishing surface, and an antibody which is connected with the surface of the gold nanorods. The gold nanorods are firstly prepared by a double surfactant method, the gold nanorods are added dropwise on the hydrophilically-treated silicon wafer polishing surface, and a continuous self assembling structure is formed by arrangement of the gold nanorods on the silicon wafer polishing surface. In the preparation process, the silicon wafer is 35 degrees to 45 degrees slantly relative to the ground, Coffee-ring Effect can be overcome, and the self-assembly gold nanorod SERS immune base has excellent SERS immune enhancing properties and repeatability, and can be sued in high sensitivity immune detection. In addition, the preparation method of the self-assembly gold nanorod SERS immune base has the advantages of simple preparation process, low cost, less time consuming and high yield, and is easy to popularize and convenient to use.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation and nanotechnology, and specifically relates to the synthesis of gold nanorods and a method for preparing a self-assembled gold nanorod SERS immune substrate. Background technique [0002] Since the discovery of the surface-enhanced Raman scattering (SERS) phenomenon, SERS spectroscopy has attracted widespread attention due to its advantages of high sensitivity and non-destructive detection, and has been developed rapidly. Using chemical or physical methods to synthesize noble metal nanoparticles and prepare them into active substrates with SERS enhancement capabilities, then SERS spectroscopy can be used to detect highly sensitive fingerprints of analytes on the surface of SERS active substrates. Therefore, it is of great significance to prepare high-performance SERS-active substrates with great SERS enhancement ability for applications in biomedical fields such as biosensing, medical imag...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N33/531
Inventor 曲兆珠周骏罗海清吴冠毅姜丹吴莎
Owner 东营睿港投资服务有限责任公司
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