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A kind of PRP/phospholipid liposome transdermal preparation and preparation method thereof

A transdermal preparation, liposome technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as no reports in research, achieve enhanced penetration, improve stability, and avoid systemic malaise effect of reaction

Active Publication Date: 2020-04-03
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, PRP is mainly prepared into a gel for use in clinical applications, and the research on encapsulating PRP with phospholipid liposomes has not been reported yet.

Method used

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  • A kind of PRP/phospholipid liposome transdermal preparation and preparation method thereof
  • A kind of PRP/phospholipid liposome transdermal preparation and preparation method thereof

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Experimental program
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preparation example Construction

[0031] In one embodiment of the present application, a kind of preparation method of PRP / phospholipid liposome transdermal preparation is provided, the steps are as follows:

[0032] (1) dissolving polyvinyl alcohol (PVA) in PBS, adding soybean lecithin, cholesterol (CHO) and methyl p-hydroxybenzoate in turn, stirring, and swelling overnight; and then undergoing ultrasonic treatment to prepare phospholipid liposomes; The ratio of polyvinyl alcohol, soybean lecithin, cholesterol and methyl p-hydroxybenzoate is (25-35): (80-100): (10-20): 1;

[0033] (2) Gradient centrifugation of autologous whole blood, that is, the first centrifugation speed is 200g, the centrifugation time is 10min, and the supernatant is taken; the second centrifugation speed is 400g, and the centrifugation time is 10min; the supernatant is discarded to obtain concentrated PRP;

[0034] (3) Inject the PRP prepared in step (2) into the phospholiposome prepared in step (1), incubate after blowing evenly, and t...

Embodiment 1

[0038] Embodiment 1: the preparation of PRP / phospholipid liposome transdermal preparation

[0039] (1) Preparation of phospholipid liposomes

[0040] Weigh 0.3 g of polyvinyl alcohol (PVA) and dissolve it in PBS (pH=5), add 0.9 g of soybean lecithin (PG90), 0.15 g of cholesterol (CHO) and 10 mg of methyl p-hydroxybenzoate in turn, stir overnight, and then Sonicate with a sonicator. 4'10" (open for 5 seconds and close for 2 seconds, cycle 50 times), 6'15" (every open for 5 seconds and close for 2 seconds, cycle 75 times), the phospholipid liposomes are prepared.

[0041] (2) Preparation of PRP:

[0042] Gradient centrifugation to obtain rat PRP: Centrifuge a certain amount of rat blood (with sodium citrate glucose solution) at 200g for 10min, take the upper layer of plasma, centrifuge at 400g for a second time for 10min, discard the supernatant, and obtain concentrated PRP.

[0043] (3) Preparation of PRP / phospholipid liposome transdermal preparation

[0044] PRP was injected...

Embodiment 2

[0045] Embodiment 2: the preparation of PRP / phospholipid liposome transdermal preparation

[0046] (1) Preparation of phospholipid liposomes

[0047] Weigh 0.3g of polyvinyl alcohol (PVA) and dissolve it in PBS (pH=5), add 0.6g of soybean lecithin (PG90), 0.3g of cholesterol (CHO) and 10mg of methyl p-hydroxybenzoate in turn, stir overnight, and then Sonicate with a sonicator. Ultrasonic time 2′5″ (every 5s on and off for 2s, cycle 25 times) to prepare phospholipid liposomes.

[0048] (2) Preparation of PRP:

[0049] Gradient centrifugation to obtain rat PRP: Centrifuge a certain amount of rat blood (with sodium citrate glucose solution) at 200g for 10min, take the upper layer of plasma, centrifuge at 400g for a second time for 10min, discard the supernatant, and obtain concentrated PRP.

[0050] (3) Preparation of PRP / phospholipid liposome transdermal preparation

[0051] PRP was injected into the aforementioned liposomes (10%, v / v) with a pipette, and incubated for 1 h (...

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Abstract

The invention discloses a preparation method of a PRP (Platelet-rich Plasma) / phospholipid liposome transdermal preparation. The preparation method comprises the following steps: (1) dissolving polyvinyl alcohol into a PBS (Phosphate Buffer Solution); sequentially adding soybean lecithin, cholesterol and methyl 4-hydroxybenzoate; stirring and swelling for one night; carrying out ultrasonic treatment to prepare phospholipid liposome; (2) carrying out gradient centrifuging on autologous whole blood to obtain concentrated PRP; (3) injecting the PRP into the phospholipid liposome; after uniformly blowing and beating, incubating to obtain the product. In the PRP / phospholipid liposome transdermal preparation, the phospholipid liposome is used as a carrier of the PRP and an active substance GFs which is continuously released by the PRP and the liposome are combined through a non-covalent bond, so that the osmosis effect of the active substance GFs and the phospholipid liposome on skin is enhanced and the active substance GFs is remained between epidermis and corium and the general side effect is avoided. A double-layered membrane of the liposome covers the PRP and the released GFs to form parcel and the PRP and the released GFs are separated from external environment, so that the stability of the active substance GFs can be extremely improved and the active substance GFs can work for a long time.

Description

technical field [0001] The invention relates to the technical field of liposome preparations, in particular to a PRP / phospholipid liposome transdermal preparation and a preparation method thereof. Background technique [0002] In today's society, people are facing increasing pressure of work and life, environmental pollution, etc., all of which will accelerate the aging of people's skin. Therefore, it is especially necessary to protect the skin and repair the damaged skin. [0003] Traditional skin care mostly uses fine chemical raw materials. Such skin care products are limited to physical (friction peeling), chemical (corrosion covering) and other effects, and cannot effectively solve the fundamental problems of the skin, let alone the aging problem of the skin. With the improvement of living standards and the rapid development of science and technology, cell science is favored in the field of dermatology, and functional cosmetics are widely concerned. Among them, biologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A61K8/14A61K8/37A61K8/55A61K8/63A61K8/81A61Q19/00
CPCA61K8/14A61K8/37A61K8/553A61K8/63A61K8/8129A61K8/983A61Q19/00
Inventor 郝海燕吴剑波沙爱龙任美萍李蓉邓鑫罗茂王立群
Owner SOUTHWEST MEDICAL UNIVERISTY