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Individualized beautifying skin care product and preparation method thereof

A technology for skin care and hirudin, which is applied in cosmetic preparations, preparations for skin care, cosmetics, etc., can solve the problems of unavoidable protein pollution with non-human impurities, allergies, etc., and achieve great promotion value and application prospects. , Enhance skin elasticity, prevent skin aging effect

Active Publication Date: 2017-07-14
江苏赛拉纳生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural hirudin extracted from traditional Chinese medicine leech is inevitably contaminated with non-human impurity protein, which has the potential risk of causing allergies, which is a disadvantageous factor for the application of natural hirudin in the field of beauty and skin care.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of lyophilized powder containing immune cell culture supernatant expressing hirudin

[0029] The preparation method of this embodiment includes steps S1-S4, which are specifically as follows.

[0030] S1. In vitro culture of personalized human CIK cells, including the collection of peripheral blood mononuclear cells (PBMC) in step A and the cultivation of CIK cells in step B.

[0031] A. The collection of PBMC has the following steps:

[0032] A1. Take 50-100ml of peripheral blood from individual customers receiving this beauty and skin care product;

[0033] A2, lymphocyte separation medium density gradient centrifugation to further purify PBMC;

[0034] A3. Wash twice with serum-free culture medium to obtain PBMCs with a purity above 90%.

[0035] B. Cell culture:

[0036] B1. Divide PBMC by 1~2×10 6 / ml concentration suspended in serum-free medium, add 1,000U / ml recombinant human IFN-γ, 37°C, 5% CO 2 Cultivated in an incubator;

[0037] B...

Embodiment 2

[0056] Embodiment 2 prepares cosmetic skin care product

[0057] Glycerin 5%, glycerol tristearate 15%, glycerol monostearate 12%, vitamin C 1%, pearl liquid 5%, cetyl alcohol 4%, sodium hyaluronate 1% and After mixing an appropriate amount of ultrapure water, heat it to 80°C to 90°C to make it fused until a soft paste appears, and then add 15% of the hirudin-expressing immune cells prepared in Example 1 when it is cooled to 30°C The culture supernatant is freeze-dried powder and 0.3% spice, and then fully stirred to make it completely uniform to obtain the beauty and skin care product.

[0058] The lyophilized powder of immune cell culture supernatant expressing hirudin in this example was prepared by the method in Example 1. Immune cells are obtained from customers who order this beauty and skin care product.

Embodiment 3

[0059] Embodiment 3 prepares cosmetic skin care product

[0060] 5% glycerin, 5% glycerol tristearate, 5% aloe extract, 5% ethanol, 15% amino acid, 0.5% salicylic acid, 5% sodium hyaluronate and an appropriate amount of ultra-pure After the water is mixed, heat it to 60°C to 70°C to make it fused, and when it is cooled to 30°C, add 12% lyophilized powder of immune cell culture supernatant expressing hirudin and 0.7% spice, and then fully stir to make it If it is completely uniform, you will get beauty and skin care products.

[0061] The lyophilized powder of immune cell culture supernatant expressing hirudin in this example was prepared by the method in Example 1. Immune cells are obtained from customers who order this beauty and skin care product.

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PUM

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Abstract

The invention discloses an individualized beautifying skin care product and a preparation method thereof. The individualized beautifying skin care product is prepared by mixing the following raw materials in percentage by weight: 1 to 20 percent of individualized human-derived immune cell culture supernatant fluid freeze-dried powder expressing hirudin, 1 to 15 percent of grease, 1 to 27 percent of aliphatic ester, 1 to 8 percent of organic solvent, 0.1 to 1 percent of perfume, 5 to 30 percent of nutrient and the balance of ultrapure water. The beautifying skin care product containing individualized immune cell culture supernatant fluid freeze-dried powder expressing the hirudin, disclosed by the invention contains most of purely natural and individualized growth factors which are required by cell growth as well as the hirudin which is transfected by genes and expressed by individualized immune cell secretion, so that immunological rejection can be avoided when the individualized beautifying skin care product is used for beautifying, and the individualized beautifying skin care product has comprehensive effects of dredging skin microcirculation, resisting microthrombus, removing spot and acne, moisturizing skin, whitening, preventing skin ageing and increasing skin elasticity, and is an individualized, efficient and green beautifying skin care product.

Description

technical field [0001] The invention relates to a biological beauty and skin care product, in particular to a personalized beauty and skin care product and a preparation method thereof. Background technique [0002] With the improvement of people's living standards, people pay more and more attention to beauty care, and there are many kinds of beauty care products on the market. However, most of the existing fine chemical skin care products contain substances that are harmful to the skin such as lead, mercury, alcohol, preservatives, hormones, spices, etc. When using beauty care products, they also cause greater damage to the skin, and even The resulting stains, red blood, allergies and other problems have caused potential threats to people's quality of life and health. [0003] Most of the existing beauty and skin care products do not contain biologically active substances. Most of the biologically active substances added to some products are made by chemical methods. Ther...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/92A61K8/64A61K8/73A61Q19/00A61Q19/02A61Q19/10
CPCA61K8/64A61K8/735A61K8/92A61K8/99A61K2800/782A61K2800/86A61Q19/00A61Q19/02A61Q19/10
Inventor 何向锋施文
Owner 江苏赛拉纳生物医药有限公司
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