Anticancer pharmaceutical composition containing vincristine
A technology of vincristine and anticancer drugs, applied in the direction of drug combinations, medical preparations containing active ingredients, antineoplastic drugs, etc., can solve the problems of unreported effects, achieve increased therapeutic effect, less toxic and side effects, and improve The effect of curative effect
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Embodiment 1
[0026] Example 1 The combined use of ZYF-38 and vincristine inhibits the proliferation of various tumor cells and normal cells
[0027] Human lung adenocarcinoma cell line A549, human normal embryonic lung cell line Wi-38 cell line, human cervical cancer cell line Hela, human breast cancer cell line MCF-7, human liver cancer cell line Bel- 7402 was rinsed with PBS, digested with 0.25% trypsin and pipetted into a single cell suspension. After routine counting, adjust the cell concentration to 4×10 4 cells / mL, inoculated in a 96-well plate at 100 µL / well. At 37°C, 5% CO 2 After culturing in a saturated humidity incubator for 24 hours, add the drug to be tested, continue to cultivate for a period of time, add 50 µL XTT-PMS, incubate for 4 hours, and detect the absorbance at 450 nm in a microplate reader.
[0028] Survival rate = (OD of experimental group - OD of blank group) / (OD of solvent control group - OD of blank group) × 100%.
[0029] Two-drug interaction coefficient ...
Embodiment 2
[0051] Example 2 The inhibitory effect of ZYF-38 combined with vincristine on transplanted tumors in mice
[0052] Male nude mice were randomly divided into control group (plus normal saline), vincristine group, ZYF-38 group and two drug combination groups, 10 in each group. First, the cancer cells with good morphology and in the logarithmic growth phase were washed with 1×PBS buffer in the ultra-clean workbench, and then washed with 0.25% trypsin + 0.02% EDTA-Na 2 Digest the cells, add 1640 medium, mix the digested cancer cells, centrifuge and collect the cancer cell pellet, then dilute the precipitated cancer cells with saline, count and adjust the cell concentration to 1.2×10 7 cells / mL, draw 0.2 mL of cell suspension with a disposable syringe and inoculate it in the armpit of the upper limb of nude mice. When the tumor grows to a size of about 1.0 cm, the drug is administered by tail vein injection. We injected normal saline 0.2mL / only into the armpit of the upper limbs ...
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