Extraction method of bovine tendon collagens

A technology of tendon collagen and extraction method, which is applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve the problems of ineffective utilization of resources, increased production cost of collagen, and low product purity, etc. Achieve the effect of easy sample recovery, high column efficiency, and high extraction rate

Inactive Publication Date: 2017-07-14
李毅 +2
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Problems solved by technology

The common purification method is simply using one-step column chromatography. This method has disadvantages such as long production cycle, low separation efficiency, and low produ

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Embodiment Construction

[0028] A method for extracting bovine Achilles tendon collagen, comprising the steps of:

[0029] 1. Pre-treatment: Weigh 100 g of fresh bovine Achilles tendon after removing soft tissue, fascia and fat, grind it into 0.2-0.5 mm-sized tissue pieces with a pulverizer, and soak in 20% NaCL for 5 minutes, with a mass fraction of 5% NaHCO 3 Soak for 30 minutes, remove serum and other components, and disinfect, and all subsequent operations will be completed under aseptic operation. The tissue pieces washed with distilled water were put into chloroform / ethanol (2:1) solution to degrease them for 4 h, and then washed 3 times with double distilled water. Fully wash with 0.05mol / L Tris-HCl buffer (pH 7.5) containing 4.5mol / L NaCl for 24h to remove components such as lipids and serum, and centrifuge at low temperature (8000r / min, 30min, 4°C); buffer solution preparation Method: Dissolve 6.06g Tris in 40 ml of ddH 2 In O, adjust the pH to 7.5 with 4mol / L HCL, add ddH 2 The total amo...

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Abstract

The invention discloses an extraction method of bovine tendon collagens. The method comprises the steps of pretreatment, extraction, precipitation, salting-out, purification, drying, and collagen preservation. The extraction method of bovine tendon collagens allows type I collagens to be separated and purified from yak tendons through using enzymatic hydrolysis and high performance liquid chromatography preparation, and has the advantages of high efficiency, high selectivity and easiness in amplification.

Description

technical field [0001] The method of the invention relates to a method for extracting bovine Achilles tendon collagen. Background technique [0002] At present, collagen is mainly extracted from pigskin, cowhide, fish skin and animal bones. Collagen derived from animal skin has a high probability of contamination, while the extraction rate of collagen derived from animal bone is low. Existing extraction systems: organic acid solution system, pepsin-acid solution system and neutral salt solution system, among which the pepsin-acid solution system has the highest extraction rate. The common purification method is simply to use one-step column chromatography. This method has the disadvantages of long production cycle, low separation efficiency, and low product purity. The long separation period and low separation efficiency not only increase the production cost of collagen, but also prevent many resources from being effectively utilized. Contents of the invention [0003] ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/36C07K1/30C07K1/14
CPCC07K14/78
Inventor 李毅王洪瑾吴晓伟
Owner 李毅
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