Method capable of synchronously detecting RT-PCR detection primer group of two melon quarantine diseases, kit and application of method

A detection kit, RT-PCR technology, applied in the direction of microorganisms, recombinant DNA technology, microorganism-based methods, etc., to achieve the effects of reducing cost and time, ensuring sensitivity and specificity, and simplifying operations
CN106947837AInactive Publication Date: 2017-07-14INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
Publication Date
2017-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of detection on melon quarantine diseases, especially relates to a method capable of synchronously and quickly detecting RT-PCR detection primer group of two melon quarantine diseases, i,e., cucumber green mottle mosaic virus (CGMMV) and acidovorax avenae subsp. citrulli (Aac), a kit and application of the method. According to the method provided by the invention, specific primers CGMMV-F / CGMMV-R, BFB-F / BFB-R are designed specifically for cucumber green mottle mosaic virus disease and acidovorax avenae subsp. Citrulli disease, and a RT-PCR detection technique is combined, synchronous detection and identification of the two diseases can be realized. The method provided by the invention is a specific, sensitive, convenient and cost-effective detection method which can synchronously and quickly detect CGMMV and Aac, can ensure the sensitivity and specificity of the detection, meanwhile, detection cost is reduced, time is saved.
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Description

technical field

[0001] The invention belongs to the technical field of detection of melon quarantine diseases, in particular to a simultaneous rapid detection of two kinds of quarantine diseases infecting melons, cucumber green mottle mosaic virus (CGMMV) and melon bacterial fruit spot disease (Aac) Methods. Background technique

[0002] Cucumber green mottle mosaic mosaic virus (CGMMV), a short-stem Virus, whose genome is positive single-stranded RNA with a full length of 6.4Kb, belongs to the genus Tobamovirus of the family Tymoviridae. The virus mainly harms Cucurbitaceae crops and seriously threatens the production of watermelon, muskmelon, gourd, etc. It is mainly transmitted by seeds for long-distance transmission, and can also be transmitted by friction contact, soil diseased residues, grafting and pollination. The disease occurred on watermelons as early as 1968 in the Kanto area of ​​Japan, causing a loss of 900 million yen in the watermelon industry; in 1978, the...

Claims

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