Pineapple leaf extract and application thereof as tyrosinase inhibitor
A technology of pineapple leaves and extracts is applied in the directions of skin care preparations, cosmetic preparations, cosmetic preparations, etc., to achieve the effect of solving resource waste and strong inhibitory effect
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Embodiment 1
[0034] Example 1 Tyrosinase Promotes the Determination of the Best Activity Conditions for L-Tyrosine to Generate Melanin
[0035] (1) Effect of temperature on the optimal activity of tyrosinase
[0036] Take 8 clean and dry brown volumetric flasks of 10mL and divide them into 4 groups, 2 in each group, and mark them as C1 and C2 respectively. Add each test solution according to Table 1:
[0037] Table 1 System composition and the addition amount of each reagent
[0038]
[0039]
[0040] Add the corresponding amount of buffer solution and L-tyrosine solution to the 4 sets of volumetric flasks according to the above table as the substrate of the reaction system, then add the corresponding amount of potato extract, and place the 4 sets of solutions at 0°C, 10°C, At 30°C and 40°C, after reacting for 35 minutes, measure the absorbance immediately (the measurement wavelength is 475nm is the best). Such as figure 1 .
[0041] (2) The influence of system reaction time on ...
Embodiment 2
[0066] (1) Take 1 kg of dried pineapple leaves, crush them, add 5 L of methanol-ethanol-isopropanol mixed solvent (the volume ratio of the three is 3:6:1), soak and extract 4 times at 40-50°C, each time The extraction time is 6 hours;
[0067] (2) merging the extracts obtained by soaking and extracting each time in step (1) to obtain the extract, and filtering to remove the solid residue to obtain the filtrate;
[0068] (3) After the filtrate obtained in step (2) was concentrated under reduced pressure to remove the organic solvent, 2.5L of water was added to disperse it, and then extracted twice with petroleum ether, using 5L of petroleum ether each time;
[0069] (4) The petroleum ether phases after the extraction of step (3) are combined, concentrated under reduced pressure, and spray-dried to obtain 3.9g of pineapple leaf extract (GC-MS such as Figure 6 ).
Embodiment 3
[0071] (1) Take 3kg of dried pineapple leaves, crush them, add 60L of methanol-ethanol-isopropanol mixed solvent (the volume ratio of the three is 3:6:1), soak and extract twice at 40-50°C, each time The extraction time is 8 hours;
[0072] (2) merging the extracts obtained by soaking and extracting each time in step (1) to obtain the extract, and filtering to remove the solid residue to obtain the filtrate;
[0073] (3) After the filtrate obtained in step (2) was concentrated under reduced pressure to remove the organic solvent, 20L of water was added to disperse it, and then extracted 4 times with petroleum ether, using 20L of petroleum ether each time;
[0074] (4) The petroleum ether phases extracted in step (3) were combined, concentrated under reduced pressure, and spray-dried to obtain 13.7 g of pineapple leaf extract.
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