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A kind of selenium-enriched fermented soybean meal, preparation method and application

A technology for fermenting soybean meal and enriching selenium, which is applied in the directions of bacteria, application, and mutant preparation used in food preparation, can solve the problems of limiting the nutritional value and retention of soybean meal, and achieves the advantages of overcoming pollution and damage, low cost and improved quality. Effect

Active Publication Date: 2020-04-14
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the related research on fermented soybean meal stays on improving the protein quality of soybean meal, and there are few reports on the research on selenium-enriched fermented soybean meal, which also limits the further improvement of the nutritional value of soybean meal

Method used

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  • A kind of selenium-enriched fermented soybean meal, preparation method and application
  • A kind of selenium-enriched fermented soybean meal, preparation method and application
  • A kind of selenium-enriched fermented soybean meal, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows:

[0040]

[0041] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0042] (1) Breeding of selenium-enriched strains

[0043] C.utilis was cultured on the YEPD culture plate for 48-72 hours, picked a single colony into a 250ml Erlenmeyer flask containing 100ml YEPD liquid medium, 180-200rpm, oscillating culture for 48-72 hours, and the bacterial liquid was centrifuged for 3-72 hours. 4 times to make a single cell suspension, so that the cell concentration was 10 8 ~10 9 individual / mL. Take 0.1 mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it in an ultra-clean workbench to air dry to form a bacterial film. in N + Ion implantation is carried out in the implanter with an energy of 14-18KeV. Six gradient doses were made for each strain,...

Embodiment 2

[0062] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows:

[0063]

[0064]

[0065] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0066] (1) Breeding of selenium-enriched strains

[0067] The specific implementation is the same as in Example 1.

[0068] (2) Preparation of seed culture solution

[0069] C. utilis cas6 was inoculated on the slant medium and cultured at 28°C for 30 hours to obtain the slant strain. The composition of the slant medium was: glucose 18g / L, peptone 18g / L, yeast extract 8g / L, selenous acid Sodium 25mg / L, agar 15g / L.

[0070] The above slant strains were inoculated into 80 mL of seed culture medium, and cultured with shaking at 28° C., 190 r / min, for 30 hours, to obtain a seed culture solution. The composition of the seed medium is: malt extract 15g / L, peptone 12g / L, yeast extract 8g / L, sodium sel...

Embodiment 3

[0086] The strain composition of the fermented soybean meal compound probiotic bacterial liquid of the present embodiment is as follows: Saccharomyces C.utilis cas65.0×10 9 cfu / ml

[0087] B. licheniforms B.Lincheniforms cas58 3.0×10 9 cfu / ml

[0088] P.pentosaceus cas138 3.0×10 9 cfu / ml

[0089] Lactococcus S.lactis cas168 2.0×10 9 cfu / ml

[0090] The preparation method of the selenium-enriched fermented soybean meal of the present embodiment is as follows:

[0091] (1) Breeding of selenium-enriched strains

[0092] The specific implementation is the same as in Example 1.

[0093] (2) Preparation of seed culture solution

[0094] C. utilis cas6 was inoculated on the slant medium and cultured at 28°C for 32 hours to obtain the slant strain. The composition of the slant medium was: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, selenous acid Sodium 30mg / L, agar 18g / L.

[0095] The above slant strains were inoculated into 80 mL of seed culture medium, and cultured ...

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Abstract

The invention discloses a selenium-enriched fermented soybean meal, a preparation method and application thereof, comprising the following steps: selecting and breeding selenium-enriched bacterial strains, preparing seed culture liquid, fermenting and cultivating, fermenting a single strain of probiotic bacteria, preparing probiotic compound bacterial liquid, and freshly cultivating Base addition, preparation of highly active selenium-enriched soybean meal. The invention adopts selenium-enriched yeast and probiotics to jointly ferment soybean meal to obtain fermented soybean meal with high organic selenium content and rich amino acid content. On the one hand, it overcomes the possible pollution and damage to the environment and animals caused by directly adding selenite substances in the feed. On the other hand, it can greatly increase the selenium content in soybean meal, which is not only rich in biological organic selenium, but also contains a large amount of Beneficial microorganisms and fermentation metabolites are more nutritious, and the quality of soybean meal is significantly improved, which can promote the digestion and absorption of nutrients by animals, enhance the immune function of the body, create a multi-functional soybean meal rich in selenium, and improve the nutritional value and efficacy of soybean meal.

Description

technical field [0001] The invention relates to a fermented soybean meal, in particular to a selenium-enriched fermented soybean meal, a preparation method and an application. Background technique [0002] Selenium is a multifunctional nutritional element necessary to maintain the normal life activities of organisms. The main manifestations of selenium deficiency in animals are white muscle disease and slow growth. The selenium content in natural food is generally low, which cannot meet the needs of normal growth of animals. Selenium-enriched feed can significantly improve animal growth performance, enhance animal reproductive performance, improve animal immune function, and improve the survival rate and disease resistance of pups. At present, the main form of supplementing selenium in animal feed is adding sodium selenite, but sodium selenite has low bioavailability, high toxicity, and is easy to pollute the environment, so its usage is strictly limited. For example, Sweden...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/01C12N1/16A23K10/12A23K10/37A23K50/30A23K50/60A23K10/26
CPCA23V2400/21A23V2400/427Y02P60/87
Inventor 吴丽芳王钰婷黄胜威蔡冬清吴正岩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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