Primer for detecting alcohol metabolizing genes by aid of pyrosequencing joint sequencing methods and application of primer

A technology of pyrosequencing and sequencing primers, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve problems such as the reduction of acetaldehyde dehydrogenase enzyme activity and the impact on the efficiency of angina pectoris, etc. Achieve the effect of good accuracy, short detection period and high throughput
CN106987623AActive Publication Date: 2017-07-28石家庄迪安医学检验实验室有限公司
13 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
石家庄迪安医学检验实验室有限公司
Publication Date
2017-07-28

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a primer for detecting alcohol metabolizing genes by the aid of pyrosequencing joint sequencing methods, and belongs to the technical field of biological detection. The primer comprises amplification primers shown as SEQ ID NO.1-4 and sequencing primers shown as SEQ ID NO.5-6. Biotin labeling is carried out by 5' ends of the primers shown as SEQ ID NO.1 and SEQ ID NO.3. The invention further discloses application of the primer to simultaneously detecting the polymorphism of SNP (single nucleotide polymorphism) loci rs671 of ALDH2 (acetaldehyde dehydrogenase 2) genes and SNP loci rs1229984 of ADH1B (alcohol dehydrogenase 1B) genes. The primer is high in detection throughput as compared with the traditional ordinary pyrosequencing with only single-SNP polymorphism detection capacity during sequencing reaction in each procedure, the detection time can be effectively shortened, and labor and the cost can be effectively reduced. Besides, the primer and the application have the advantages of accurate detection results, good specificity, high sensitivity, short detection cycle, simplicity in operation and capability of meeting clinical examination requirements.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a method and primers for detecting alcohol metabolism genes by pyrosequencing combined sequencing. Background technique

[0002] The acetaldehyde dehydrogenase encoded by the ALDH2 gene and the alcohol dehydrogenase encoded by the ADH1B gene are the key enzymes in the process of alcohol metabolism. The substance is further converted into harmless acetic acid (the component of vinegar) under the catalysis of acetaldehyde dehydrogenase. The polymorphism of the coding gene causes the change of the catalytic ability of the enzyme, which will affect the metabolism of alcohol and cause the accumulation of harmful substances such as acetaldehyde, which will affect the health of the body. The G1510A polymorphism (rs671) in the ALDH2 gene will cause the glutamic acid at position 487 of the amino acid sequence to be replaced by lysine (ie, Glu487Lys), and the wild...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More