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Urine cerebrospinal fluid total protection detection reagent for fully automatic biochemical analyzer

A biochemical analyzer and detection reagent technology, applied in the field of urine, cerebrospinal fluid total protein detection, to solve the effects of factors such as urobilinogen and chyle, and increase the solubility

Inactive Publication Date: 2017-07-28
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In view of the above problems, the present invention provides a urine cerebrospinal fluid total protein detection reagent for a full-automatic biochemical analyzer, which adopts succinic acid-sodium benzoate buffer solution and adds methanol to the reagent to increase the solubility and stability of pyrogallol red. sensitivity and sensitivity, using a double-reagent formula, which effectively solves the influence of factors such as urobilinogen and chyle, and can effectively solve the problems in the background technology

Method used

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  • Urine cerebrospinal fluid total protection detection reagent for fully automatic biochemical analyzer
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  • Urine cerebrospinal fluid total protection detection reagent for fully automatic biochemical analyzer

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Embodiment 1

[0033] The present invention provides a urine cerebrospinal fluid total protein detection reagent for an automatic biochemical analyzer. The reagent is composed of two reagents, buffer R1 and chromogenic liquid R2, and its components are as follows:

[0034] R1: methanol 10%

[0035] Sodium benzoate 2.5mmol / L

[0036] Succinic acid 50mmol / L

[0037] R2: Pyrogallol Red 240umol / L

[0038] Sodium molybdate 140umol / L.

[0039] The reagent uses the red complex formed by pyrogallol red and molybdic acid in the color developing solution R2, and combines with protein under acidic conditions to form a blue-violet complex. The absorbance is positively correlated with the protein content in the sample. , Calculate the protein concentration; the maximum absorption wavelength of the red complex is 470nm; the maximum absorption wavelength of the blue-violet complex is 604nm; the pH value of the acidic condition is 2.5.

Embodiment 2

[0041] The present invention provides a urine cerebrospinal fluid total protein detection reagent for an automatic biochemical analyzer. The reagent is composed of two reagents, buffer R1 and chromogenic liquid R2, and its components are as follows:

[0042] R1: methanol 11%

[0043] Sodium benzoate 2.6mmol / L

[0044] Succinic acid 52mmol / L

[0045] R2: Pyrogallol Red 245umol / L

[0046] Sodium molybdate 142umol / L.

[0047] The reagent uses the red complex formed by pyrogallol red and molybdic acid in the color developing solution R2, and combines with protein under acidic conditions to form a blue-violet complex. The absorbance is positively correlated with the protein content in the sample. , Calculate the protein concentration; the maximum absorption wavelength of the red complex is 470nm; the maximum absorption wavelength of the blue-violet complex is 604nm; the pH value of the acidic condition is 2.5.

Embodiment 3

[0049] Detectable range: take the national standard material (human serum albumin component standard material, BW3651, 5mg / bottle), and dilute to 5g / L, 4.5g / L, 4.0g / L, 3.5g / L, 3.0g / L, 2.5g / L, 2.0g / L, 1.5g / L, 1.0g / L, 0.5g / L, 0.4g / L, 0.3g / L, 0.2g / L, 0.1g / L, 0.05g / L, There are 19 concentrations of 0.04g / L, 0.03g / L, 0.02g / L, 0.01g / L. The detection system was calibrated with 1.0g / L as the standard, and 19 standards of concentration were measured. The results are as follows.

[0050] Standard concentration (g / L) Test result (g / L) Offset (%) 154.52-9.60 24.54.132-8.18 344.0220.55 43.53.487-0.37 533.0311.03 62.52.5542.16 721.981-0.95 81.51.5080.53 910.981-1.90 100.50.5081.60 110.40.395-1.25 120.30.298-0.67 130.20.2021.00 140.10.096-4.00 150.050.0512.00 160.040.0412.50 170.030.025-16.67 180.020.011-45.00 190.010.005-50.00

[0051] It can be seen that the protein concentration is between 0.040 and 4.00 g / L, showing good linearity. As attached figure 1 .

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Abstract

The invention discloses a urine cerebrospinal fluid total protection detection reagent for a fully automatic biochemical analyzer. The urine cerebrospinal fluid total protection detection reagent comprises two reagents such as a buffer liquid R1 and a coloring liquid R2, wherein the buffer liquid R1 comprises 10-12% of methanol, 2.5-2.7 mmol / L sodium benzoate and 50-54 mmol / L succinic acid, and the coloring liquid R2 comprises 240-250 [mu]mol / L pyrogallol red and 140-145 [mu]mol / L sodium molybdate. According to the present invention, the succinic acid-sodium benzoate buffer liquid is used, and methanol is added to the reagent so as to increase the solubility, the stability and the sensitivity of pyrogallol red; and by using the double reagent formula, the influence of urobilinogen, chyle and other factors is effectively solved.

Description

Technical field [0001] The invention relates to the technical field of urine and cerebrospinal fluid total protein detection, in particular to a urine and cerebrospinal fluid total protein detection reagent for an automatic biochemical analyzer. Background technique [0002] Protein is an important substance indispensable for life metabolism. [0003] Cerebrospinal fluid proteins are formed by blood filtering through the blood-brain barrier into the cerebral ventricles, subarachnoid space and central canal of the spinal cord, or synthesized by the central nervous system. The total protein of normal cerebrospinal fluid is not more than 0.42g / L. When the blood-brain barrier is destroyed or multiple sclerosis and other diseases, when the central nervous system autoimmune globulin synthesis increases, the total cerebrospinal fluid protein increases. [0004] Under the action of the normal glomerular charge barrier and membrane barrier, a small amount of albumin enters the first urine. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/314
Inventor 张建张义张晓时
Owner SHANDONG UNIV QILU HOSPITAL