Multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead, and preparation and application thereof

A technology of soybean protein isolate and sodium alginate, which is applied in the field of soybean protein isolate composite materials, can solve problems such as complex preparation process, achieve good swelling performance, increase drug loading and encapsulation efficiency, and good swelling performance

Active Publication Date: 2017-08-04
潍坊希普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of the above gels uses organic synthesis reagents

Method used

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  • Multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead, and preparation and application thereof
  • Multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead, and preparation and application thereof
  • Multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Take a certain amount of soybean protein isolate and disperse it in 1.5mol / L NaOH solution to obtain a soybean protein isolate dispersion with a content of 0.01g / mL.

[0044] (2) Take 10mL of soybean protein isolate dispersion (containing 0.1g of soybean protein isolate), add 5mL of sodium alginate aqueous solution (concentration: 0.01g / mL, containing 0.05g of sodium alginate) to it, stir and mix evenly at room temperature to obtain Soy Protein Isolate / Sodium Alginate Blend.

[0045] (3) Slowly drip the mixture of soybean protein isolate / sodium alginate into 50mL of calcium chloride methanol solution (concentration: 0.1mol / L) and let it stand for 80 min; then add 20mL of sodium hexametaphosphate aqueous solution (concentration 1 mmol / L, containing sodium hexametaphosphate 0.0184g), pour off the solvent after the cross-linking reaction at room temperature for 50 minutes, separate the gel beads, and wash with distilled water until the eluate is neutral, dry the free w...

Embodiment 2

[0048] (1) Take a certain amount of isolated soybean protein and disperse it into a KOH solution with a concentration of 1.5 mol / L to obtain a soybean protein isolate dispersion with a content of 0.05 g / mL.

[0049] (2) Take 10mL of soybean protein isolate dispersion (containing 0.5g of soybean protein isolate), add 5mL of sodium alginate aqueous solution (concentration: 0.1 g / mL, containing 0.5g of sodium alginate), and stir and mix evenly at room temperature to obtain Soy Protein Isolate / Sodium Alginate Blend.

[0050] (3) Slowly instill the soybean protein isolate / sodium alginate mixture into 25 mL of calcium chloride in methanol solution (concentration 0.7 mol / L), and let it stand for 20 min; then add 15 mL of sodium hexametaphosphate aqueous solution (concentration 10 mmol / L, containing 0.0918g sodium hexametaphosphate), after the cross-linking reaction at room temperature for 10 minutes, the solvent was poured out, the gel beads were separated, and washed with distilled ...

Embodiment 3

[0053] (1) Take a certain amount of isolated soybean protein and disperse it into a NaOH solution with a concentration of 1.0mol / L to obtain a soybean protein isolate dispersion with a content of 0.1g / mL.

[0054] (2) Take 10mL of soybean protein isolate dispersion (containing 1g of soybean protein isolate), add 40mL of sodium alginate aqueous solution (concentration: 0.05 g / mL, containing 2g of sodium alginate), and stir and mix evenly at room temperature to obtain soybean isolate protein / sodium alginate mixture.

[0055] (3) Slowly drip the soybean protein isolate / sodium alginate mixture into 40mL calcium chloride methanol solution (concentration 0.4mol / L), let it stand for 50 min; then add 30mL containing 1-ethyl-3- Aqueous solution of (3-dimethylaminopropyl) carbodiimide hydrochloride (concentration 5 mmol / L, containing 0.0288 g of cross-linking agent), after cross-linking reaction at room temperature for 30 minutes, the solvent was poured out, and the gel beads were separ...

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Abstract

The invention discloses a multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead. The compound hydrogel bead is obtained by taking soybean protein isolate and sodium alginate as raw materials through a non-toxic and biocompatible crosslinking agent. The compound hydrogel bead has good swelling property, responsiveness, ionic strength sensitivity and biodegradability. A drug loading property test verifies that compared with a sodium alginate hydrogel bead, the soybean protein isolate compounded hydrogel bead is greatly improved in drug loading capacity and encapsulation efficiency of drug molecules, and the swelling property is better. A drug release property test verifies that the cumulative release rate of the soybean protein isolate compounded hydrogel bead on the drug is obviously greater than that of the sodium alginate hydrogel bead. Therefore, the multi-sensitive soybean protein isolate-sodium alginate compound hydrogel bead can be applied to the medical field as a macromolecular drug carrier.

Description

technical field [0001] The invention relates to a soybean protein isolate composite material, in particular to the preparation of a soybean protein isolate-sodium alginate composite microgel beads; the invention also relates to the soybean protein isolate-sodium alginate composite gel beads as a drug carrier Application; belongs to the fields of composite materials, edible natural polymer materials and medicinal polymer materials. Background technique [0002] Among the medical polymer carrier materials, new drug carrier materials occupy a very important position. During the use of the drug, choosing an appropriate carrier material will greatly improve the effectiveness of the drug. An ideal drug carrier should have good drug release performance, good biocompatibility, biodegradability, physical, chemical and biological stability and extremely low cytotoxicity. In recent years, different kinds of drug carrier materials have been developed. In order to improve biocompatibi...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L89/00C08L5/04A61K47/42A61K47/36
CPCA61K47/36A61K47/42C08J3/246C08J2305/04C08J2389/00C08J2405/04C08J2489/00C08L5/04C08L89/00
Inventor 何玉凤钱立忠闫桂芳王荣民宋鹏飞
Owner 潍坊希普生物科技有限公司
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